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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AM05261PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM05261PU-N, RRID:AB_1618767
- Product name
- anti LEF1 (HMG Binding Domain)
- Antibody type
- Monoclonal
- Antigen
- Full-length recombinant LEF-1 protein.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- REMB6
- Vial size
- 0.1 mg
- Concentration
- Lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis using LEF-1/TCF antibody (AM05261PU) on Jurkat cell lysate (AS00010LY-N) at 5 µg/ml.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Cos-1 cells transfected with (from Top Down) LEF-1, LEF-1 mutant missing nuclear localization signal, TCF-4 and TCF-1. Left column shows AM05261PU-N HMG/TCF antibody, (Clone REMB6) visualized with FITC anti-Mouse. Right column is DAPI staining to detect all nuclei.
