Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA324545 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal ARNT Antibody (Center V528)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal ARNT Antibody (Center V528)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ARNT
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 2.0 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ARNT Antibody (V528) (Cat. #TA324545) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ARNT antibody detected the ARNT protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of A549 cell stained with hARNT-V528(Cat#TA324545).A549 cells were incubated with hARNT primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml).hARNT immunoreactivity is localized to Nucleus significantly.
- Validation comment
- IF
