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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00000405-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000405-M01, RRID:AB_536922
- Product name
- Mouse Monoclonal ARNT/HIF-1 beta Antibody
- Antibody type
- Monoclonal
- Description
- ARNT/HIF-1 beta
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARNT/HIF-1 beta Antibody (3D10) [H00000405-M01] - Analysis of ARNT over-expressed 293 cell line, cotransfected with ARNT Validated Chimera RNAi ( Cat # H00000405-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ARNT monoclonal antibody (M01), clone 3D10 (Cat # H00000405-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARNT/HIF-1 beta Antibody (3D10) [H00000405-M01] - Analysis of ARNT expression in transfected 293T cell line by ARNT monoclonal antibody (M01), clone 3D10.Lane 1: ARNT transfected lysate(86.6 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARNT/HIF-1 beta Antibody (3D10) [H00000405-M01] - ARNT monoclonal antibody (M01), clone 3D10 Analysis of ARNT expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ARNT/HIF-1 beta Antibody (3D10) [H00000405-M01] - Detection limit for recombinant GST tagged ARNT is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody (3D10) [H00000405-M01] - Analysis of monoclonal antibody to ARNT on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ARNT/HIF-1 beta Antibody (3D10) [H00000405-M01] - Analysis of monoclonal antibody to ARNT on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.
