AF6988

antibody from Novus Biologicals

Targeting: FOXF2 FKHL6, FREAC2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6988

Provider

Novus Biologicals

Product name

Sheep Polyclonal FoxF2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human FoxF2 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) FoxF1, rhFoxP3, and rhFoxC2 is observed.

Reactivity

Human

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Hedgehog signaling patterns the oral-aboral axis of the mandibular arch.

Xu J, Liu H, Lan Y, Adam M, Clouthier DE, Potter S, Jiang R
eLife 2019 Jan 14;8

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human FoxF2 by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human FoxF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6988) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxF2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.