PA5-11889
antibody from Invitrogen Antibodies
Targeting: NEUROD1
BETA2, BHF-1, bHLHa3, MODY6, NEUROD
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-11889 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NeuroD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Neurogenic Potential of the 18-kDa Mitochondrial Translocator Protein (TSPO) in Pluripotent P19 Stem Cells.
González-Blanco L, Bermejo-Millo JC, Oliveira G, Potes Y, Antuña E, Menéndez-Valle I, Vega-Naredo I, Coto-Montes A, Caballero B
Cells 2021 Oct 17;10(10)
Cells 2021 Oct 17;10(10)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a NeuroD1 polyclonal antibody (Product # PA5-11889) in Y79 cell line, mouse cerebellum and rat brain tissue lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 ( A ) Markers of pluripotency and neuronal differentiation in undifferentiated P19 stem cells (SC) and cells treated with 1 muM retinoid acid (RA) or 50 muM PK 11195 (PK 11195) during 4 days of treatment. ( B ) Bar chart shows quantification of the optical densities (OD) from three independent cell cultures and treatments. Data are expressed as means +- SEM. ** p < 0.01 versus SC; *** p < 0.001 versus SC; # p < 0.05 versus RA; ## p < 0.01 versus RA; ### p < 0.001 versus RA. Protein shows a representative ponceau staining of one of the experiments. The complete ponceau staining for each antibody is shown in the Supplementary Figure S1 . Although the entire membrane of ponceau S was used for total protein normalization, only a representative section of the membrane is displayed in this image.