IC2085G

antibody from Novus Biologicals

Targeting: TBXT T

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC2085G

Provider

Novus Biologicals

Product name

Goat Polyclonal Brachyury Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed.

Reactivity

Human, Mouse

Host

Goat

Conjugate

Green dye

Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

References

Submitted references

Single-cell qPCR demonstrates that Repsox treatment changes cell fate from endoderm to neuroectoderm and disrupts epithelial-mesenchymal transition.

Li Q, Huang Q
PloS one 2019;14(10):e0223724

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Generation of heterozygous and homozygous hESC H9 sublines carrying inactivating mutations in RB1.

Schipper L, Kanber D, Steenpass L
Stem cell research 2018 Dec;33:41-45

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Brachyury in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line differentiated with mouse serum for 4 days was stained with Goat Anti-Human/Mouse Brachyury Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.