MA5-29556

antibody from Invitrogen Antibodies

Targeting: SERPINB1 anti-elastase, EI, ELANH2, PI2

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29556

Provider

Invitrogen Antibodies

Product name

SERPINB1 Monoclonal Antibody (5)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Antibody clone number

5

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SERPINB1 in Lane A: NCI-H460 Whole Cell Lysate (30 µg). Samples were probed using a SERPINB1 Monoclonal Antibody (Product # MA5-29556) at a 1:500 dilution, followed by a Goat Anti-Mouse IgG (H+L), Dylight 800 Secondary Antibody at a 1:15000 dilution. Western blot was performed under reducing conditions. Predicted band size:43 kDa. Observed band size:43 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SERPINB1 Monoclonal Antibody (5) (Product # MA5-29556) at 1:500 dilution. Lane A: NCI-H460 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG H&L (DyLight™ 800) at 1:15,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SERPINB1 Immunoprecipitation using: Lane A: 0.5 mg 293T Whole Cell Lysate 0.5 µL with SERPINB1 Monoclonal Antibody (5) (Product # MA5-29556) and 15 µL of 50 % Protein G agarose. Primary antibody: SERPINB1 Monoclonal Antibody (5), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7,500 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa.