Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-79770 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HNF6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HNF6 in Lane 1: rat liver tissue lysate, Lane 2: mouse liver tissue lysate, Lane 3: HeLa cell lysate. Sample was incubated with HNF6 polyclonal antibody (Product # PA5-79770).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ONECUT1 in, Lane 1: human HepG2 whole cell lysates, Lane 2: human HUH7 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse liver tissue lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. The membrane was blocked with 5% non-fat milk/TBS for 1. 5 hour at RT. The membrane was incubated with HNF6 Polyclonal Antibody (Product # PA5-79770) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0. 1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for ONECUT1 at approximately 55 kDa. The expected band size for ONECUT1 is at 51 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-HNF6 Polyclonal Antibody(Product # PA5-79770) and a 52 kDa band corresponding to HNF6 was observed across the cell lines and tissues tested. Whole cell extracts of Hep G2 (Lane 1), SK-O-V3 (Lane 2), NIH: OVCAR-3 (Lane 3), HeLa (Lane 4), Mouse Liver (Lane 5), Rat Liver (Lane 6) and Mouse Heart (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Hep G2 is a high expressing model and SK-O-V3 and NIH: OVCAR-3 are Low expressing cell models. In Tissues, Liver is a high expressing model whereas Heart is a low expressing model for Onecut1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of HNF6 in HCT116 cells. Sample was incubated with HNF6 polyclonal antibody (Product # PA5-79770).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HNF6 on frozen rat liver tissue. Sample was incubated with HNF6 polyclonal antibody (Product# PA5-79770).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HNF6 on paraffin-embedded human liver cancer tissue. Sample was incubated with HNF6 polyclonal antibody (Product# PA5-79770).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HNF6 on paraffin-embedded rat liver tissue. Sample was incubated with HNF6 polyclonal antibody (Product# PA5-79770).