Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- 710233 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RUNX1 Recombinant Polyclonal Antibody (3HCLC)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine and non-human primate based on sequence homology.
- Antibody clone number
- 3HCLC
- Concentration
- 0.5 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of PC-3 (Lane 1), K562 (Lane 2), THP-1 (Lane 3) and Mouse Small Intestine (Lane 4). The blots were probed with Recombinant Rabbit Polyclonal Anti-RUNX1 Antibody (Product # 710233, 1-3 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Two bands ~ 60 and 48 kDa band corresponding to RUNX1 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a transferred onto a nitrocellulose membrane with Pierce™ Power Blotter System (22834). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RUNX1 in fetal mouse thymus lysate using a RUNX1 Recombinant Rabbit Polyclonal Antibody (Product # 710233) at a dilution of 1 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~52kDa.