PA5-20739

antibody from Invitrogen Antibodies

Targeting: RUNX1 AML1, AMLCR1, CBFA2, PEBP2A2

Antibody data

Submit

Validation data

RUNX1 protein structure - PA5-20739 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Raji cell lysate using a RUNX1 polyclonal antibody (Product # PA5-20739) at (A) 1 and (B) 2 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of RUNX1 in Raji cell lysate with RUNX1 Polyclonal Antibody (Product # PA5-20739) at (A) 1 and (B) 2 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of RUNX1 was achieved by transfecting Jurkat with RUNX1 specific siRNAs (Silencer® select Product # s229351, s2458). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the RUNX1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with RUNX1 Polyclonal Antibody (Product # PA5-20739, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RUNX1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-RUNX1 Polyclonal Antibody, (Product # PA5-20739) and 55 kDa, 28 kDa bands corresponding to RUNX1 isoforms along with an uncharacterized band (*) at ~80 kDa was observed in the cell lines tested. Modified whole cell extracts (1% SDS) (40 µg lysate) of Jurkat (Lane 1), THP-1 (Lane 2), HEL 92.1.7 (Lane 3), MCF7 (Lane 4), T-47D (Lane 5) and IMR32 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).