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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00007280-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00007280-M03, RRID:AB_1137068
- Product name
- TUBB2A monoclonal antibody (M03), clone 2B2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant TUBB2A.
- Antigen sequence
MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGS
YHGDSDLQLERINVYYNEAAGNKYVPRAILVDLEP
GTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGH
YTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLG
GGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKV
SDTVVEPYNATLSVHQLVENTDETYSIDNEALYDI
CFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQ
LNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQ
YRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAA
IFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK
TAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQF
TAMFRRKAFLHWYTGEGMDEMEFTEAESNTNDLVS
EYQQYQDATADEQGEFEEEEGEDEA- Isotype
- IgG
- Antibody clone number
- 2B2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of TUBB2A expression in transfected 293T cell line by TUBB2A monoclonal antibody (M03), clone 2B2.Lane 1: TUBB2A transfected lysate(49.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- TUBB2A monoclonal antibody (M03), clone 2B2. Western Blot analysis of TUBB2A expression in Raw 264.7(Cat # L024V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to TUBB2A on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to TUBB2A on formalin-fixed paraffin-embedded human spleen. [antibody concentration 1.5 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
