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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [23]
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- Product number
- UM800054 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM800054, RRID:AB_2629165
- Product name
- Vimentin (VIM) mouse monoclonal antibody,clone UMAB159
- Antibody type
- Monoclonal
- Description
- Vimentin (VIM) mouse monoclonal antibody,clone UMAB159
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- VIM
- Isotype
- IgG
- Antibody clone number
- UMAB159
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Colon, 9: Spleen, 10: Thyroid). Diluation: 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-Vimentin mouse monoclonal antibody (UM800054, green, 1:100). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human glioma using anti-vimentin clone UMAB159 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800054 requires HIER with with Accel 3in1 EDTA solution pH8.7 at 110C for 3 min using pressure chamber/cooker. The neural cells shows membrane and cytoplasmic staining.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of two case of paraffin-embedded human ovarain cancer using anti-Vimentin clone UMAB159 mouse mAb (UM800054) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human tonsil using anti-Vimentin clone UMAB159 mouse monoclonal antibody (UM800054) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer usin
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human three cases of gastric cancer using anti-Vimentin clone UMAB159 mouse monoclonal antibody (UM800054) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human placenta using anti-Vimentin clone UMAB159 mouse monoclonal antibody (UM800054) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer us
- Validation comment
- IHC
