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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- IC2105A - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Vimentin APC-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Vimentin in Western blots. Unconjugated antibody detects mouse and rat Vimentin in immunocytochemistry.
- Reactivity
- Human, Mouse, Rat
- Host
- Rat
- Conjugate
- Red dye
- Antigen sequence
P08670- Isotype
- IgG
- Antibody clone number
- 280618
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references New therapeutic opportunities from dissecting the pre-B leukemia bone marrow microenvironment.
Cheung LC, Tickner J, Hughes AM, Skut P, Howlett M, Foley B, Oommen J, Wells JE, He B, Singh S, Chua GA, Ford J, Mullighan CG, Kotecha RS, Kees UR
Leukemia 2018 Nov;32(11):2326-2338
Leukemia 2018 Nov;32(11):2326-2338
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Vimentin in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Rat Anti-Human/Mouse/Rat Vimentin APC-conjugated Monoclonal Antibody (Catalog # IC2105A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
