Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-29907 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FHL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299, mouse brain.
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FHL2 using 30 µg of H1299 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a FHL2 polyclonal antibody (Product # PA5-29907) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FHL2 was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a FHL2 Polyclonal Antibody (Product # PA5-29907) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FHL2 Polyclonal Antibody (Product # PA5-29907). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. FHL2 Polyclonal Antibody (Product # PA5-29907) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SAS xenograft , using FHL2 (Product # PA5-29907) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.