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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- GTX50039 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX50039, RRID:AB_11177372
- Product name
- AVEN antibody [3G4]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- The extracts of mouse kidney (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD12 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Paraffin embedded sections of human breast lobule tissue were incubated with anti-human PDCD12 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
