TA505014

antibody from OriGene

Targeting: MAFB KRML

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA505014

Provider

OriGene

Proper citation

OriGene Cat#TA505014, RRID:AB_2622765

Product name

MAFB mouse monoclonal antibody, clone OTI3F6 (formerly 3F6)

Antibody type

Monoclonal

Description

MAFB mouse monoclonal antibody, clone OTI3F6 (formerly 3F6)

Host

Mouse

Conjugate

Unconjugated

Epitope

MAFB

Isotype

IgG

Antibody clone number

OTI3F6

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAFB (RC207311, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAFB.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAFB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC207311 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MAFB antibody(TA505014), and then analyzed by flow cytometry.

Validation comment

FC