MA1-41076

antibody from Invitrogen Antibodies

Targeting: TNFRSF10A Apo2, CD261, DR4, TRAILR-1, TRAILR1

Flow cytometry

Antibody data

Submit

Validation data

Product number: MA1-41076 - Provider product page
Provider: Invitrogen Antibodies
Product name: DR4 Monoclonal Antibody (32A1380)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: Suggested positive control: Daudi.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 32A1380
Vial size: 100 µg
Concentration: 1.0 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

TNFRSF10A protein structure - MA1-41076 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of DR4 in Daudi cell lysate using a CD261/TRAIL Receptor 1 DR4 monoclonal antibody (Product # MA1-41076) at 2 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of DR4 in Daudi cell lysate. Samples were incubated in DR4 monoclonal antibody (Product # MA1-41076) using a dilution of 2 µg/mL followed by a goat anti-mouse HRP secondary antibody. PicoTect ECL substrate solution was used for this test.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of DR4 in 0.5 mg/mL Daudi lysate. Samples were incubated in DR4 monoclonal antibody (Product # MA1-41076). This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot was performed using Anti-DR4 Monoclonal Antibody (32A1380) (Product # MA1-41076) and a 54 kDa band corresponding to Tumor necrosis factor receptor superfamily member 10A was observed across tested samples. Membrane enriched extracts (40 µg lysate) of MDA-MB-231 (Lane 1), T-47D (Lane 2), MCF7 (Lane 3), A549 (Lane 4), HeLa (Lane 5), Caco-2 (Lane 6), IMR-32 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (0.5 µg/mL concentration) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).