Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ARP42202_T100 - Provider product page
- Provider
- Aviva Systems Biology
- Proper citation
- Aviva Systems Biology Cat#ARP42202_T100, RRID:AB_937708
- Product name
- ACTN4 antibody - N-terminal region (ARP42202_T100)
- Antibody type
- Polyclonal
- Antigen
- The immunogen is a synthetic peptide directed towards the N terminal region of human ACTN4
- Description
- Protein A purified
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Horse, Rabbit, Zebrafish
- Host
- Rabbit
- Vial size
- 100 μl
- Concentration
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Storage
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis using Anti-ACTN4 antibody detects protein at predicted molecular weight using Hct116 Cells as a positive control. Tissue/Lysate Concentration: 0.625ug/ml Antibody Concentration: 1.0ug/ml
- Sample type
- Hct116 Cells
- Validation comment
- Western blot analysis detects protein at predicted molecular weight
- Primary Ab dilution
- 1.0ug/ml
- Other comments
- Lysate concentration 0.625ug/ml
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Sample Type : ACTNX-GFP transfected COS-7 cellsPrimary Antibody Dilution : 1:1000Secondary Antibody : Anti-rabbit-Alexa-Fluor 568Secondary Antibody Dilution : 1:100Color/Signal Descriptions : Green: GFP Red: ACTN4Gene Name : ACTN4Submitted by : Johannes W. Hell, Ph.D., Professor, Department of Pharmacology, University of California, Room 2419D Tupper Hall, Davis, CA 95616-5270, Phone: (530) 752 6540, Lab: (530) 752 7551, FAX: (530) 752 7710