- PA5-22259 - Invitrogen Antibodies ACTN4 antibody

No submitted references: Submit reference

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using alpha Actinin 4 Polyclonal Antibody (Product # PA5-22259). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 7.5% SDS PAGE. alpha Actinin 4 Polyclonal Antibody (Product # PA5-22259) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: alpha Actinin 4 Polyclonal Antibody detects alpha Actinin 4 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract. 7.5% SDS-PAGE. alpha Actinin 4 Polyclonal Antibody (Product # PA5-22259) dilution: 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using alpha Actinin 4 Polyclonal Antibody (Product # PA5-22259). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 7.5% SDS PAGE. alpha Actinin 4 Polyclonal Antibody (Product # PA5-22259) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using alpha Actinin 4 Polyclonal Antibody (Product # PA5-22259). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: HepG2. 7.5% SDS PAGE. alpha Actinin 4 Polyclonal Antibody (Product # PA5-22259) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot was performed using Anti-alpha Actinin 4 Polyclonal Antibody (Product # PA5-22259) and a ~110 kDa band corresponding to Alpha-actinin-4 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), A-431 (Lane 3), IMR-90 (Lane 4), PANC-1 (Lane 5), DU 145 (Lane 6), LNCaP (Lane 7), PC-12 (Lane 8) and NIH/3T3 (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). https://doi.org/10.1016/j.bbrc.2019.12.084

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of alpha Actinin 4 in paraformaldehyde-fixed HeLa cells using an Alpha Actinin 4 polyclonal antibody (Product # PA5-22259) at a 1:200 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Alpha-actinin-4 was performed using 70% confluent log phase DU 145 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with alpha Actinin 4 Polyclonal Antibody (Product # PA5-22259) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Nucleus and cytoplasm localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4 (Product # PA5-22259) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PA5-22259