PA5-27249

antibody from Invitrogen Antibodies

Targeting: CST4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27249

Provider

Invitrogen Antibodies

Product name

Cystatin S Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Cystatin S using 30 µg of HepG2 lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a Cystatin S polyclonal antibody (Product # PA5-27249) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Cystatin S Polyclonal Antibody (Product # PA5-27249). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cystatin S Polyclonal Antibody (Product # PA5-27249) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Cystatin S Polyclonal Antibody (Product # PA5-27249). Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cystatin S Polyclonal Antibody (Product # PA5-27249) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Cystatin S in methanol-fixed A431 cells using a Cystatin S polyclonal antibody (Product # PA5-27249) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Cystatin S (Product # PA5-27249) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.