Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA1-06100 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Laminin Monoclonal Antibody (A5)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-06100 detects laminin in human and mouse samples.
- Antibody clone number
- A5
- Concentration
- 1 mg/mL
Submitted references A 50 kdyne contusion spinal cord injury with or without the drug SS-31 was not associated with major changes in muscle mass or gene expression 14 d after injury in young male mice.
Skeletal muscle transcriptional networks linked to type I myofiber grouping in Parkinson's disease.
Cell-Based Strain Remodeling of a Nonfibrous Matrix as an Organizing Principle for Vasculogenesis.
Disease-modifying bioactivity of intravenous cardiosphere-derived cells and exosomes in mdx mice.
The role of pericytic laminin in blood brain barrier integrity maintenance.
Deletion of muscle GRP94 impairs both muscle and body growth by inhibiting local IGF production.
Anti-DNA autoantibodies initiate experimental lupus nephritis by binding directly to the glomerular basement membrane in mice.
Blood-brain barrier disruption in CCL2 transgenic mice during pertussis toxin-induced brain inflammation.
Omega 3 fatty acids supplementation has an ameliorative effect in experimental ulcerative colitis despite increased colonic neutrophil infiltration.
Regulation of muscle mass by follistatin and activins.
Diaphragm displays early and progressive functional deficits in dysferlin-deficient mice.
Decellularized rhesus monkey kidney as a three-dimensional scaffold for renal tissue engineering.
Expression profiling reveals heightened apoptosis and supports fiber size economy in the murine muscles of mastication.
Coupling of neural activity to blood flow in olfactory glomeruli is mediated by astrocytic pathways.
Altered renal morphology in transgenic mice with cholecystokinin overexpression.
Misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance.
Expression and distribution of tissue transglutaminase in normal and injured rat cornea.
Globose basal cells are required for reconstitution of olfactory epithelium after methyl bromide lesion.
Graham ZA, DeBerry JJ, Cardozo CP, Bamman MM
Physiological reports 2021 Feb;9(4):e14751
Physiological reports 2021 Feb;9(4):e14751
Skeletal muscle transcriptional networks linked to type I myofiber grouping in Parkinson's disease.
Lavin KM, Sealfon SC, McDonald MN, Roberts BM, Wilk K, Nair VD, Ge Y, Lakshman Kumar P, Windham ST, Bamman MM
Journal of applied physiology (Bethesda, Md. : 1985) 2020 Feb 1;128(2):229-240
Journal of applied physiology (Bethesda, Md. : 1985) 2020 Feb 1;128(2):229-240
Cell-Based Strain Remodeling of a Nonfibrous Matrix as an Organizing Principle for Vasculogenesis.
Rüdiger D, Kick K, Goychuk A, Vollmar AM, Frey E, Zahler S
Cell reports 2020 Aug 11;32(6):108015
Cell reports 2020 Aug 11;32(6):108015
Disease-modifying bioactivity of intravenous cardiosphere-derived cells and exosomes in mdx mice.
Rogers RG, Fournier M, Sanchez L, Ibrahim AG, Aminzadeh MA, Lewis MI, Marbán E
JCI insight 2019 Apr 4;4(7)
JCI insight 2019 Apr 4;4(7)
The role of pericytic laminin in blood brain barrier integrity maintenance.
Gautam J, Zhang X, Yao Y
Scientific reports 2016 Nov 3;6:36450
Scientific reports 2016 Nov 3;6:36450
Deletion of muscle GRP94 impairs both muscle and body growth by inhibiting local IGF production.
Barton ER, Park S, James JK, Makarewich CA, Philippou A, Eletto D, Lei H, Brisson B, Ostrovsky O, Li Z, Argon Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2012 Sep;26(9):3691-702
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2012 Sep;26(9):3691-702
Anti-DNA autoantibodies initiate experimental lupus nephritis by binding directly to the glomerular basement membrane in mice.
Krishnan MR, Wang C, Marion TN
Kidney international 2012 Jul;82(2):184-92
Kidney international 2012 Jul;82(2):184-92
Blood-brain barrier disruption in CCL2 transgenic mice during pertussis toxin-induced brain inflammation.
Schellenberg AE, Buist R, Del Bigio MR, Toft-Hansen H, Khorooshi R, Owens T, Peeling J
Fluids and barriers of the CNS 2012 Apr 30;9(1):10
Fluids and barriers of the CNS 2012 Apr 30;9(1):10
Omega 3 fatty acids supplementation has an ameliorative effect in experimental ulcerative colitis despite increased colonic neutrophil infiltration.
Varnalidis I, Ioannidis O, Karamanavi E, Ampas Z, Poutahidis T, Taitzoglou I, Paraskevas G, Botsios D
Revista espanola de enfermedades digestivas 2011 Oct;103(10):511-8
Revista espanola de enfermedades digestivas 2011 Oct;103(10):511-8
Regulation of muscle mass by follistatin and activins.
Lee SJ, Lee YS, Zimmers TA, Soleimani A, Matzuk MM, Tsuchida K, Cohn RD, Barton ER
Molecular endocrinology (Baltimore, Md.) 2010 Oct;24(10):1998-2008
Molecular endocrinology (Baltimore, Md.) 2010 Oct;24(10):1998-2008
Diaphragm displays early and progressive functional deficits in dysferlin-deficient mice.
Barton ER, Wang BJ, Brisson BK, Sweeney HL
Muscle & nerve 2010 Jul;42(1):22-9
Muscle & nerve 2010 Jul;42(1):22-9
Decellularized rhesus monkey kidney as a three-dimensional scaffold for renal tissue engineering.
Nakayama KH, Batchelder CA, Lee CI, Tarantal AF
Tissue engineering. Part A 2010 Jul;16(7):2207-16
Tissue engineering. Part A 2010 Jul;16(7):2207-16
Expression profiling reveals heightened apoptosis and supports fiber size economy in the murine muscles of mastication.
Evans M, Morine K, Kulkarni C, Barton ER
Physiological genomics 2008 Sep 17;35(1):86-95
Physiological genomics 2008 Sep 17;35(1):86-95
Coupling of neural activity to blood flow in olfactory glomeruli is mediated by astrocytic pathways.
Petzold GC, Albeanu DF, Sato TF, Murthy VN
Neuron 2008 Jun 26;58(6):897-910
Neuron 2008 Jun 26;58(6):897-910
Altered renal morphology in transgenic mice with cholecystokinin overexpression.
Aunapuu M, Roosaar P, Järveots T, Kurrikoff K, Kõks S, Vasar E, Arend A
Transgenic research 2008 Dec;17(6):1079-89
Transgenic research 2008 Dec;17(6):1079-89
Misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance.
Kotani T, Kawakami K
Developmental biology 2008 Apr 15;316(2):383-96
Developmental biology 2008 Apr 15;316(2):383-96
Expression and distribution of tissue transglutaminase in normal and injured rat cornea.
Zhang W, Shiraishi A, Suzuki A, Zheng X, Kodama T, Ohashi Y
Current eye research 2004 Jan;28(1):37-45
Current eye research 2004 Jan;28(1):37-45
Globose basal cells are required for reconstitution of olfactory epithelium after methyl bromide lesion.
Jang W, Youngentob SL, Schwob JE
The Journal of comparative neurology 2003 May 19;460(1):123-40
The Journal of comparative neurology 2003 May 19;460(1):123-40
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of laminin was performed using 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Laminin Monoclonal Antibody (Product # MA1-06100) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rat IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Laminin beta 2 gamma 1 in frozen sections from human colon (methanol fixed) using a Laminin beta-2/gamma-1 Monoclonal Antibody (A5) (Product # MA1-06100), showing the localization of laminin in the connective tissue and in basement membranes surrounding the epithelial crypts and villi.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Laminin beta 2 gamma 1 in frozen sections from pig striated skeletal muscle (methanol fixed) using a Laminin beta-2/gamma-1 Monoclonal Antibody (A5) (Product # MA1-06100), showing the localization of laminin in the connective tissue and in basement membranes surrounding the myofibrils.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Laminin beta 2 gamma 1 in human kidney frozen tissue section using a Laminin beta-2/gamma-1 Monoclonal Antibody (A5) (Product # MA1-06100), diluted 1:100.
