LS-B3427

antibody from LSBio

Targeting: ACO2 ACONM

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B3427

Provider

LSBio

Proper citation

LifeSpan Cat#LS-B3427, RRID:AB_2221510

Product name

IHC-plus™ ACO2 / Aconitase 2 Antibody (aa438-467) LS-B3427

Antibody type

Polyclonal

Description

Ammonium sulfate precipitation

Reactivity

Human, Mouse, Rat

Host

Rabbit

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of anti-ACO2 antibody in mouse heart(left) and 293 (right) tissue lysates (35 ug/lane). ACO2(arrow) was detected using the purified antibody.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail (Sigma); PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2. Solution: 1:1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4 ?. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

ACO2 western blot of 293 cell line lysates (35 ug/lane). The Aconitase antibody detected the Aconitase protein (arrow).