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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C178 - Provider product page
- Provider
- LSBio
- Product name
- ALOX5 / 5-LOX Antibody (phospho-Ser523) LS-C178
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Rat
- Host
- Rabbit
- Storage
- Short term: -20°C; Long term: -20°C.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of rat cortex lysate showing specific immunolabeling of the ~80k doublet of 5-LO phosphorylated at Ser523 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser523 5-LO. The immunolabeling is completely eliminated by treatment with l-Ptase.
