LS-C18771

antibody from LSBio

Targeting: CDK1 CDC2, CDC28A

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C18771

Provider

LSBio

Product name

CDK1 / CDC2 Antibody (C-Terminus) LS-C18771

Antibody type

Polyclonal

Description

Delipidated and defibrinated

Reactivity

Human, Mouse, Rat

Host

Rabbit

Storage

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-cdc2 Cyclin Dependent Kinase was used to detect human p34cdc2by Western blot of untreated (Control) and drug treated lysates of MCF-7 cells. Lane 1-4 represents 3.1 mM, 6.2 mM, 12.5 mM and 25.0 mM genistein treatment of cells before lysates were prepared. Detection occurs using a 1:1000 dilution. Although this antiserum detects non-specific bands at higher MW, a clear induction of signal is observed as the concentration of drug is increased.