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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- LS-C18772 - Provider product page
- Provider
- LSBio
- Product name
- CDK1 / CDC2 Antibody (clone POH-1) LS-C18772
- Antibody type
- Monoclonal
- Description
- Protein A purified
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- POH-1
- Storage
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Mab anti-Human p34cdc2 antibody was used at a dilution of 1:1000 to detect human p34cdc2by Western blot of untreated (control) and drug treated (10 mM genistein) lysates of MCF-7 cells.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Mab anti-Human p34°Cdc2 antibody (clone POH-1) is shown to detect human p34°Cdc2by western blot. Detection occurs after 10 ug of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1000 dilution of Mab anti-Human p34°Cdc2at room temperature for 30 min followed by detection using IRDye800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:5000. A doublet band corresponding to human p34°Cdc2is detected at ~34 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human p34°Cdc2. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Mab anti-Human p34Cdc2 antibody was used to detect human p34Cdc2by Western blot of untreated (control) and drug treated (10 M genistein) lysates of MCF-7 cells. Very strong detection occurs using a 1:1000 dilution. Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center.
