LS-C18954

antibody from LSBio

Targeting: ALB

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

LS-C18954

Provider

LSBio

Product name

ALB / Serum Albumin Antibody (HRP) LS-C18954

Antibody type

Polyclonal

Description

Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification.

Reactivity

Human

Host

Rabbit

Conjugate

Horseradish Peroxidase

Isotype

IgG

Storage

Store vial at 4°C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Secondary Antibodies - Western Blot. secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein ( 250 ng starting total load) co incubated with LS-C60865 and 1:20K in MB-070. Blot was dried and imaged (A) on Bio-Rad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager,. This image was taken for the unconjugated form of this product. Other forms have not been tested.