LS-C98842

antibody from LSBio

Targeting: BCL2 Bcl-2, PPP1R50

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C98842

Provider

LSBio

Proper citation

LifeSpan Cat#LS-C98842, RRID:AB_2274728

Product name

BCL2 / Bcl-2 Antibody (aa75-110) LS-C98842

Antibody type

Polyclonal

Description

Ammonium sulfate precipitation

Reactivity

Human, Rat

Host

Rabbit

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Left panel, of Bcl-2 BH3 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) by using this antibody for immunoprecipitation (IP) and a different Bcl-2 antibody for western blot. Right panel, same analysis using antibody in flow cytometry. Data courtesy Dr Quan Chen, Chinese Academy of Sciences.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Formalin-fixed and paraffin-embedded human testis tissue reacted with Bcl-2 BH3 Domain Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Formalin-fixed and paraffin-embedded human testis tissue reacted with Bcl-2 BH3 Domain Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.