10326-1-AP

antibody from Proteintech Group

Targeting: TRIM32 BBS11, HT2A, LGMD2H, TATIP

Provider product page for 10326-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [19]
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Validations
Western blot [1]

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Product number: 10326-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10326-1-AP, RRID:AB_2208005
Product name: TRIM32 antibody
Antibody type: Polyclonal
Description: KD/KO validated TRIM32 antibody (Cat. #10326-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

TRIM32 protein structure - 10326-1-AP shown in red.

Submitted references Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.

Balakrishnan K, Chakraborty S, Chiang C, Stratton CM, Pandey S, Tumanova AA, Olsen SK, Gack MU
Proceedings of the National Academy of Sciences of the United States of America 2026 Mar 31;123(13):e2528398123

TRIM32 promotes anoikis resistance and metastasis in NSCLC by degrading CHEK2 to enhance IL-6 secretion.

Xu J, Liu W, Chen L, Zhang J, Zhang D, Huang X, Zhang G
Cell death and differentiation 2026 Jan;33(1):126-139

TRIM32 promotes neuronal ferroptosis by enhancing K63-linked ubiquitination and subsequent p62-selective autophagic degradation of GPX4.

Zhou X, Zhao Y, Huang S, Shu H, Zhang Y, Yang H, Ren Y, Zhou X, Liu W, Song T, Zhao J, Ma J
International journal of biological sciences 2025;21(3):1259-1274

Inhibition of coronaviral exonuclease activity by TRIM-mediated SUMOylation.

Balakrishnan K, Chakraborty S, Chiang C, Stratton C, Tumanova AA, Olsen SK, Gack MU
bioRxiv : the preprint server for biology 2025 Jul 29;

Small intestinal γδ T17 cells promote SAE through STING/C1q-induced microglial synaptic pruning in male mice.

Wu Y, Zhang Y, Yu Y, Wang X, Zhen Z, Yuan Y, Xie B, Han M, Wang M, Zhang X, Sun X, Wen X, Hashimoto K, Shang Y, Yuan S, Zhang J
Nature communications 2025 Jul 23;16(1):6779

Activity-dependent phosphorylation of CDYL by CDK5 regulates fear memory in mice.

Lyu NY, Xie GG, Hu ZW, Lyu TJ, Qin R, Chen L, Wang Y
Translational psychiatry 2025 Aug 30;15(1):334

TRIM32 inhibits Venezuelan equine encephalitis virus infection by targeting a late step in viral entry.

Xie Y, Cao J, Gan S, Xu L, Zhang D, Qian S, Xu F, Ding Q, Schoggins JW, Fan W
PLoS pathogens 2024 Nov;20(11):e1012312

TRIM32 inhibits Venezuelan Equine Encephalitis Virus Infection by targeting a late step in viral entry.

Xie Y, Cao J, Gan S, Xu L, Zhang D, Qian S, Xu F, Ding Q, Schoggins JW, Fan W
bioRxiv : the preprint server for biology 2024 Jun 4;

Opposing regulation of the STING pathway in hepatic stellate cells by NBR1 and p62 determines the progression of hepatocellular carcinoma.

Nishimura S, Linares JF, L'Hermitte A, Duran A, Cid-Diaz T, Martinez-Ordoñez A, Ruiz-Martinez M, Kudo Y, Marzio A, Heikenwalder M, Roberts LR, Diaz-Meco MT, Moscat J
Molecular cell 2024 Dec 5;84(23):4660-4676.e10

Ubiquitin ligase TRIM32 promotes dendrite arborization by mediating degradation of the epigenetic factor CDYL.

Liu L, Liu TT, Xie GG, Zhu XQ, Wang Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022 Jan;36(1):e22087

Generation of the short TRIM32 isoform is regulated by Lys 247 acetylation and a PEST sequence.

Garcia-Garcia J, Overå KS, Khan W, Sjøttem E
PloS one 2021;16(5):e0251279

Deficiency of TRIM32 Impairs Motor Function and Purkinje Cells in Mid-Aged Mice.

Zhu JW, Jia WQ, Zhou H, Li YF, Zou MM, Wang ZT, Wu BS, Xu RX
Frontiers in aging neuroscience 2021;13:697494

An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.

Wilson MM, Callens C, Le Gallo M, Mironov S, Ding Q, Salamagnon A, Chavarria TE, Viel R, Peasah AD, Bhutkar A, Martin S, Godey F, Tas P, Kang HS, Juin PP, Jetten AM, Visvader JE, Weinberg RA, Attanasio M, Prigent C, Lees JA, Guen VJ
Science advances 2021 Oct 29;7(44):eabf6063

A nonautophagic role of ATG5 in regulating cell growth by targeting c-Myc for proteasome-mediated degradation.

Li S, Zhang L, Zhang G, Shangguan G, Hou X, Duan W, Xi Y, Xu N, Zhang B, Dong J, Wang Y, Cui W, Chen S
iScience 2021 Nov 19;24(11):103296

Knockdown of TRIM32 inhibits tumor growth and increases the therapeutic sensitivity to temozolomide in glioma in a p53-dependent and -independent manner.

Cai Y, Gu WT, Cheng K, Jia PF, Li F, Wang M, Zhang WF, Qiu JT, Wu ZB, Zhao WG
Biochemical and biophysical research communications 2021 Apr 23;550:134-141

Proteome Analysis of Isolated Podocytes Reveals Stress Responses in Glomerular Sclerosis.

Koehler S, Kuczkowski A, Kuehne L, Jüngst C, Hoehne M, Grahammer F, Eddy S, Kretzler M, Beck BB, Höhfeld J, Schermer B, Benzing T, Brinkkoetter PT, Rinschen MM
Journal of the American Society of Nephrology : JASN 2020 Mar;31(3):544-559

TRIM32, but not its muscular dystrophy-associated mutant, positively regulates and is targeted to autophagic degradation by p62/SQSTM1.

Overå KS, Garcia-Garcia J, Bhujabal Z, Jain A, Øvervatn A, Larsen KB, Deretic V, Johansen T, Lamark T, Sjøttem E
Journal of cell science 2019 Dec 2;132(23)

TRIM32 promotes cell proliferation and invasion by activating β-catenin signalling in gastric cancer.

Wang C, Xu J, Fu H, Zhang Y, Zhang X, Yang D, Zhu Z, Wei Z, Hu Z, Yan R, Cai Q
Journal of cellular and molecular medicine 2018 Oct;22(10):5020-5028

Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component.

Kudryashova E, Wu J, Havton LA, Spencer MJ
Human molecular genetics 2009 Apr 1;18(7):1353-67

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 10326-1-AP(TRIM32 antibody) at dilution of 1:500
Sample type: cell line