11200-1-AP

antibody from Proteintech Group

Targeting: BCAP31 6C6-Ag, BAP31, CDM, DXS1357E

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

11200-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#11200-1-AP, RRID:AB_2065142

Product name

BAP31 antibody

Antibody type

Polyclonal

Description

KD/KO validated BAP31 antibody (Cat. #11200-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

BAP31 Modulates Mitochondrial Homeostasis Through PINK1/Parkin Pathway in MPTP Parkinsonism Mouse Models.

Zhang W, Meng S, Hao Z, Zhu X, Cao L, Yuan Q, Wang B
Cells 2026 Jan 12;15(2)

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Pathogenic mechanisms of amyotrophic lateral sclerosis-linked VAPB P56S mutation in the degeneration of corticospinal motor neurons.

Yang X, Zheng J, Wang X, Cai H, Yu J
Ageing and neurodegenerative diseases 2025;5(3)

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BAP31 represses endoplasmic reticulum stress-mediated apoptosis and alleviates neurodegeneration in Parkinson's disease.

Qin Y, Chen Y, Chi M, Jia J, Wang L, Zhao Y, Wang Z, Zhou J, Yao J
Cell death & disease 2025 Sep 29;16(1):672

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Deacetylation of BAP31 by sirtuin 2 attenuates apoptosis of hepatocytes induced by endoplasmic reticulum stress, in chronic alcoholic liver injury.

Wang S, Wang Y, Shan W, Li G, Yan R, Wang Z, Zhao Y, Yao J, Zhang N
British journal of pharmacology 2025 Oct;182(20):5023-5037

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Upregulated BAP31 Links to Poor Prognosis and Tumor Immune Microenvironment in Breast Cancer.

Hao Z, Zhao B, Zhu X, Zhang W, Wang B
International journal of molecular sciences 2025 Jun 21;26(13)

---

Genomewide Screen Identifies Peroxisomal Role in APOL1 Podocytopathy.

Kim J, Karel IZ, Song H, Dewalt M, Orwick S, Buelow DR, Lee K, Brodsky SV, Blissett A, Cocucci E, Baker SD, Lin PH, Pabla NS, Madhavan SM
medRxiv : the preprint server for health sciences 2025 Feb 18;

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ARCN1 suppresses innate immune responses against respiratory syncytial virus by promoting STUB1-mediated IKKε degradation.

Cai J, Ye Y, Chen Z, Xu F, Li X, Wu M, Zhou J, Shao Y, Zhu P, Zhao J, Hu J, Wang Y, Li C, Tian X, Huang B, Xia T, Wang W, Hao C, Yang Y, Zhang J
PLoS pathogens 2025 Dec;21(12):e1013751

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B-Cell Receptor-Associated Protein 31 Deficiency Aggravates Ethanol-Induced Liver Steatosis and Liver Injury via Attenuating Fatty Acid Oxidation and Glycogen Synthesis.

Yu S, Xia Y, Zhang C, Han X, Feng X, Li L, Ma H, Xu J
International journal of molecular sciences 2025 Dec 18;26(24)

---

The EMC acts as a chaperone for membrane proteins.

Klose CJ, Meighen-Berger KM, Kulke M, Parr M, Steigenberger B, Zacharias M, Frishman D, Feige MJ
Nature communications 2025 Aug 2;16(1):7097

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Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling.

Kalarikkal M, Saikia R, Oliveira L, Bhorkar Y, Lonare A, Varshney P, Dhamale P, Majumdar A, Joseph J
EMBO reports 2024 Oct;25(10):4226-4251

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BAP31 Promotes Angiogenesis via Galectin-3 Upregulation in Neuroblastoma.

Namusamba M, Wu Y, Yang J, Zhang Q, Wang C, Wang T, Wang B
International journal of molecular sciences 2024 Mar 3;25(5)

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Interactome Analysis Identifies the Role of BZW2 in Promoting Endoplasmic Reticulum-Mitochondria Contact and Mitochondrial Metabolism.

Maio G, Smith M, Bhawal R, Zhang S, Baskin JM, Li J, Lin H
Molecular & cellular proteomics : MCP 2024 Feb;23(2):100709

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Subcellular mRNA kinetic modeling reveals nuclear retention as rate-limiting.

Steinbrecht D, Minia I, Milek M, Meisig J, Blüthgen N, Landthaler M
Molecular systems biology 2024 Dec;20(12):1346-1371

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An endoplasmic reticulum stress related signature for clinically predicting prognosis of breast cancer patients.

Qiao E, Ye J, Huang K
Human molecular genetics 2024 Dec 4;

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Legionella uses host Rab GTPases and BAP31 to create a unique ER niche.

Chadha A, Yanai Y, Oide H, Wakana Y, Inoue H, Saha S, Paul M, Tagaya M, Arasaki K, Mukherjee S
Cell reports 2024 Dec 24;43(12):115053

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Rapid nuclear deadenylation of mammalian messenger RNA.

Alles J, Legnini I, Pacelli M, Rajewsky N
iScience 2023 Jan 20;26(1):105878

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BAP31 regulates the expression of ICAM-1/VCAM-1 via MyD88/NF-κB pathway in acute lung injury mice model.

Li G, Jiang X, Liang X, Hou Y, Zang J, Zhu B, Jia C, Niu K, Liu X, Xu X, Jiang R, Wang B
Life sciences 2023 Jan 15;313:121310

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Interaction of species A rotavirus VP4 with the cellular proteins vimentin and actin related protein 2 discovered by a proximity interactome assay.

Hao P, Qu Q, Pang Z, Li L, Du S, Shang L, Jin C, Xu W, Ha Z, Jiang Y, Chen J, Gao Z, Jin N, Wang J, Li C
Journal of virology 2023 Dec 21;97(12):e0137623

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Mammalian oocytes store mRNAs in a mitochondria-associated membraneless compartment.

Cheng S, Altmeppen G, So C, Welp LM, Penir S, Ruhwedel T, Menelaou K, Harasimov K, Stützer A, Blayney M, Elder K, Möbius W, Urlaub H, Schuh M
Science (New York, N.Y.) 2022 Oct 21;378(6617):eabq4835

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B-cell receptor associated protein 31 deficiency decreases the expression of adhesion molecule CD11b/CD18 and PSGL-1 in neutrophils to ameliorate acute lung injury.

Li GX, Jiang XH, Zang JN, Zhu BZ, Jia CC, Niu KW, Liu X, Jiang R, Wang B
The international journal of biochemistry & cell biology 2022 Nov;152:106299

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HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins.

Zinnall U, Milek M, Minia I, Vieira-Vieira CH, Müller S, Mastrobuoni G, Hazapis OG, Del Giudice S, Schwefel D, Bley N, Voigt F, Chao JA, Kempa S, Hüttelmaier S, Selbach M, Landthaler M
Nature communications 2022 May 18;13(1):2727

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Co-translational biogenesis of lipid droplet integral membrane proteins.

Leznicki P, Schneider HO, Harvey JV, Shi WQ, High S
Journal of cell science 2022 Mar 1;135(5)

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Caffeic acid phenethyl ester protects against doxorubicin-induced cardiotoxicity and increases chemotherapeutic efficacy by regulating the unfolded protein response.

Zhang Y, Kong D, Han H, Cao Y, Zhu H, Cui G
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2022 Jan;159:112770

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Calcineurin Controls Cellular Prion Protein Expression in Mouse Astrocytes.

Dematteis G, Restelli E, Vanella VV, Manfredi M, Marengo E, Corazzari M, Genazzani AA, Chiesa R, Lim D, Tapella L
Cells 2022 Feb 10;11(4)

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BAP31 Promotes Tumor Cell Proliferation by Stabilizing SERPINE2 in Hepatocellular Carcinoma.

Zhang X, Jiang D, Yang S, Sun Y, Liu Y, Shi J, Hu C, Pan J, Liu T, Jin B, Yang K
Frontiers in cell and developmental biology 2020;8:607906

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Proteomic analyses reveal that Orf virus induces the activation and maturation of mouse bone marrow-derived dendritic cells.

Feng Q, Li L, Du G, Liu Y, Liu X, Wu J, Shang Y
Research in veterinary science 2020 Oct;132:563-573

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Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress.

Merriman C, Fu D
The Journal of biological chemistry 2019 Nov 8;294(45):16992-17006

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MiR-451a suppressing BAP31 can inhibit proliferation and increase apoptosis through inducing ER stress in colorectal cancer.

Xu K, Han B, Bai Y, Ma XY, Ji ZN, Xiong Y, Miao SK, Zhang YY, Zhou LM
Cell death & disease 2019 Feb 15;10(3):152

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Efficient proximity labeling in living cells and organisms with TurboID.

Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, Feldman JL, Perrimon N, Ting AY
Nature biotechnology 2018 Oct;36(9):880-887

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Expansion of DUB functionality generated by alternative isoforms - USP35, a case study.

Leznicki P, Natarajan J, Bader G, Spevak W, Schlattl A, Abdul Rehman SA, Pathak D, Weidlich S, Zoephel A, Bordone MC, Barbosa-Morais NL, Boehmelt G, Kulathu Y
Journal of cell science 2018 May 16;131(10)

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FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.

Wu W, Lin C, Wu K, Jiang L, Wang X, Li W, Zhuang H, Zhang X, Chen H, Li S, Yang Y, Lu Y, Wang J, Zhu R, Zhang L, Sui S, Tan N, Zhao B, Zhang J, Li L, Feng D
The EMBO journal 2016 Jul 1;35(13):1368-84

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Directed evolution of APEX2 for electron microscopy and proximity labeling.

Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY
Nature methods 2015 Jan;12(1):51-4

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Fis1 and Bap31 bridge the mitochondria-ER interface to establish a platform for apoptosis induction.

Iwasawa R, Mahul-Mellier AL, Datler C, Pazarentzos E, Grimm S
The EMBO journal 2011 Feb 2;30(3):556-68

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11200-1-AP(BCAP31 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using BCAP31 antibody 11200-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-BCAP31 (IP:11200-1-AP, 3ug; Detection:11200-1-AP 1:1000) with A431 cells lysate 3300ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human colon cancer using 11200-1-AP(BCAP31 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Flow cytometry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The BAP31 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human BAP31. This antibody recognizes human,mouse,rat antigen. The BAP31 antibody has been validated for the following applications: ELISA, WB, IHC, IF, IP, FC analysis.

Appendix

11200-1-AP.pdf

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