12384-1-AP

antibody from Proteintech Group

Targeting: UCHL3

Provider product page for 12384-1-AP

Western blot

ELISA

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [21]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 12384-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12384-1-AP, RRID:AB_2210915
Product name: UCHL3 antibody
Antibody type: Polyclonal
Description: KD/KO validated UCHL3 antibody (Cat. #12384-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

UCHL3 protein structure - 12384-1-AP shown in red.

Submitted references A TRIM21-UCHL3-ITCH-SIPA1 axis promotes colorectal cancer growth and metastasis.

Lou S, Wang H, Mo G, Li H, Xie H, Huang Y, Li H, Ma K, Zhang X, Yan M, Zhang J, Pi Y, Han P
Cancer letters 2026 Feb 28;639:218221

UCHL3 Promotes Triple-Negative Breast Cancer Metastatic Potential Through Enhancing Cell Migration and Invasion.

Xu Q, Yang W, Li C, Yang M, Zhu X, Wang W, Lin Y, Gan Z, He T, Wu X, Li X
Applied biochemistry and biotechnology 2026 Apr 7;

UCHL3 augments cuproptosis via PKM2 deubiquitination in hepatocellular carcinoma.

Yao L, Ye S, Xu D
Free radical biology & medicine 2025 Sep;237:65-75

Expression and role of deubiquitinating enzymes in thyroid carcinoma.

Huang M, Yan C, Ling R, Wang T
Briefings in functional genomics 2025 Jan 15;24

MLKL-USP7-UBA52 signaling is indispensable for autophagy in brain through maintaining ubiquitin homeostasis.

Zhang Z, Chen S, Jun S, Xu X, Hong Y, Yang X, Zou L, Song YQ, Chen Y, Tu J
Autophagy 2025 Feb;21(2):424-446

Site-Specific Nanobody Inhibitors of the Proteasomal Deubiquitinase UCH37.

Li Y, Chang LH, Patel R, Shestoperova EI, Du J, Das C, Strieter ER
bioRxiv : the preprint server for biology 2025 Aug 13;

Quantification Quality Control Emerges as a Crucial Factor to Enhance Single-Cell Proteomics Data Analysis.

Yu SH, Chen SC, Wu PS, Kuo PI, Chen TA, Lee HY, Lin MH
Molecular & cellular proteomics : MCP 2024 May;23(5):100768

GSK3β and UCHL3 govern RIPK4 homeostasis via deubiquitination to enhance tumor metastasis in ovarian cancer.

Shan W, Peng W, Chen Y, Wang Y, Yu Q, Tian Y, Dou Y, Tu J, Huang X, Li X, Wang Z, Zhu Q, Chen J, Xia B
Oncogene 2024 Jun;43(25):1885-1899

UCHL3 promotes hepatocellular carcinoma progression by stabilizing EEF1A1 through deubiquitination.

Zhao J, Huo Q, Zhang J, Sun K, Guo J, Cheng F, Hu X, Xu Q
Biology direct 2024 Jul 4;19(1):53

CircMTA2 Drives Gastric Cancer Progression through Suppressing MTA2 Degradation via Interacting with UCHL3.

Xie G, Lei B, Yin Z, Xu F, Liu X
International journal of molecular sciences 2024 Feb 29;25(5)

UCHL3 inhibits ferroptosis by stabilizing β-catenin and maintains stem-like properties of hepatocellular carcinoma cells.

Long G, Wu Z, Wang D, Mi X, Hu K, Zhou L, Tang J
Free radical biology & medicine 2024 Feb 20;212:162-173

Porphyromonas gingivalis induced UCHL3 to promote colon cancer progression.

Lu Y, Huang R, Zhang Y, Xiang W, Zhang X, Chen F, An L, Yuan H, Wen F, Xu Y
American journal of cancer research 2023;13(12):5981-5995

UCHL3 promotes hepatocellular carcinoma cell migration by de-ubiquitinating and stabilizing Vimentin.

Ma Q, Lu Q, Lei X, Zhao J, Sun W, Wang J, Zhu Q, Huang D
Frontiers in oncology 2023;13:1088475

The deubiquitinating enzyme UCHL3 promotes anaplastic thyroid cancer progression and metastasis through Hippo signaling pathway.

Tang J, Yang Q, Mao C, Xiao D, Liu S, Xiao L, Zhou L, Wu G, Tao Y
Cell death and differentiation 2023 May;30(5):1247-1259

Farrerol directly activates the deubiqutinase UCHL3 to promote DNA repair and reprogramming when mediated by somatic cell nuclear transfer.

Zhang W, Wang M, Song Z, Fu Q, Chen J, Zhang W, Gao S, Sun X, Yang G, Zhang Q, Yang J, Tang H, Wang H, Kou X, Wang H, Mao Z, Xu X, Gao S, Jiang Y
Nature communications 2023 Apr 3;14(1):1838

Structural basis for specific inhibition of the deubiquitinase UCHL1.

Grethe C, Schmidt M, Kipka GM, O'Dea R, Gallant K, Janning P, Gersch M
Nature communications 2022 Oct 10;13(1):5950

Ubiquitin C-terminal hydrolase L1 (UCHL1) regulates post-myocardial infarction cardiac fibrosis through glucose-regulated protein of 78 kDa (GRP78).

Lei Q, Yi T, Li H, Yan Z, Lv Z, Li G, Wang Y
Scientific reports 2020 Jun 30;10(1):10604

UCHL3 promotes pancreatic cancer progression and chemo-resistance through FOXM1 stabilization.

Song Z, Li J, Zhang L, Deng J, Fang Z, Xiang X, Xiong J
American journal of cancer research 2019;9(9):1970-1981

The deubiquitylating enzyme UCHL3 regulates Ku80 retention at sites of DNA damage.

Nishi R, Wijnhoven PWG, Kimura Y, Matsui M, Konietzny R, Wu Q, Nakamura K, Blundell TL, Kessler BM
Scientific reports 2018 Dec 17;8(1):17891

An Interaction Landscape of Ubiquitin Signaling.

Zhang X, Smits AH, van Tilburg GB, Jansen PW, Makowski MM, Ovaa H, Vermeulen M
Molecular cell 2017 Mar 2;65(5):941-955.e8

A phosphorylation-deubiquitination cascade regulates the BRCA2-RAD51 axis in homologous recombination.

Luo K, Li L, Li Y, Wu C, Yin Y, Chen Y, Deng M, Nowsheen S, Yuan J, Lou Z
Genes & development 2016 Dec 1;30(23):2581-2595

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 12384-1-AP(UCHL3 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The UCHL3 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human UCHL3. This antibody recognizes human,mouse,rat antigen. The UCHL3 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 12384-1-AP.pdf

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