20415-1-AP

antibody from Proteintech Group

Targeting: MIF GIF, GLIF

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

20415-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#20415-1-AP, RRID:AB_10694820

Product name

MIF antibody

Antibody type

Polyclonal

Description

KD/KO validated MIF antibody (Cat. #20415-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, rat, mouse and has been validated for the following applications: FC, IF, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

S-nitrosation in endothelium: different outcomes of NRF2 and GSNOR loss.

Kopacz A, Kloska D, Kraszewska I, Targosz-Korecka M, Kubisiak A, Jankowska U, Skupien-Rabian B, Grochot-Przeczek A, Jozkowicz A
Free radical biology & medicine 2026 Feb 16;244:323-335

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Targeting keratin 6 A overcomes gemcitabine resistance by restoring equilibrative nucleoside transporter 1 and TAM-mediated metabolic compensation in pancreatic cancer.

Zhang J, Wang X, Zeng S, Tang B, Yang X, Xie S, Sun H, Wang R, Xia R, Li J, Wang H, Shi Z, Gu J, Wei D
Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy 2026 Apr 17;87:101404

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Multi-omics integration reveals that pyrimidine metabolism in lung adenocarcinoma drives an immunosuppressive microenvironment.

Liu M, Li H, Xu S, Zhou Y, Yao M, Shi J, Zhong L
iScience 2026 Apr 17;29(4):115326

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MIF-Mediated NLRP3 Inflammasome-Dependent Pyroptosis in Spinal Neurons and Microglial Polarization Facilitate Neuropathic Pain Progression.

Zhou F, Tian Y, Liao W, Ma Q, Bao H, Meng F, Jiang J
Anesthesiology research and practice 2025;2025:6624776

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Single-Cell Transcriptomic Analysis Highlights the Impact of NFKB2-Mediated MIF-CD44 Signaling Axis in Endometrioid Endometrial Cancer.

Zhang L, Yang M, Zhang Q, Zhang Y, Li Q, Chen Q
International journal of women's health 2025;17:3293-3313

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Identification of a stromal immunosuppressive barrier orchestrated by SPP1(+)/C1QC(+) macrophages and CD8(+) exhausted T cells driving gastric cancer immunotherapy resistance.

Ma G, Liu X, Jiang Q, Li S, Wu Q, Liang B, Sun F, Gu C, Liao W, Zhang Z, Shi M, Huang Q
Frontiers in immunology 2025;16:1618591

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Increased ocular plasma cells induce damaging α-synuclein(+) microglia in autoimmune uveitis.

Li M, Feng M, Liu T, Duan S, Man X, Yuan X, Wang L, Sun Y, Wei X, Fu Q, Sun B, Lin W
Mucosal immunology 2025 Jun;18(3):668-684

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Single-cell RNA sequencing analysis revealed the immunosuppressive remodeling of tumor-associated macrophages mediated by the MIF-CD74 axis in gastric cancer.

Liu W, Wang C, Liang L, Zhang C, Li Y, Xiao J, Zeng R, Li J, Lin Z, Huang Q, Zheng L, Zhang Q
Scientific reports 2025 Jul 24;15(1):26883

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Single-Cell Transcriptomic Analysis Reveals Biomechanical Loading-Induced Imbalance in Bone and Fat, Leading to Ossification in Lumbar Intervertebral Disc Nucleus Pulposus Degeneration.

Zhang P, Wang Y, Bai J, Zhang J, Zhang S, Guo X, Zhan J, Zhu L
Journal of cellular physiology 2025 Jan;240(1):e31506

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Targeting macrophage migration inhibitory factor to inhibit T cell immunosuppression in the tumor microenvironment and improve cancer outcomes in head and neck squamous cell carcinoma.

Pracha SH, Shrestha S, Ryan N, Upadhaya P, Lamenza FF, Jagadeesha S, Jordanides P, Roth P, Springer A, Oghumu S
Oral oncology 2025 Jan;160:107126

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CDR1as Deficiency Prevents Photoreceptor Degeneration by Regulating miR-7a-5p/α-syn/Parthanatos Pathway in Retinal Detachment.

Jin F, Yan Y, Ye Z, Wang L, Deng C, Jiang J, Dong K
The American journal of pathology 2025 Feb;195(2):293-305

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Single-cell transcriptome analysis identifies MIF as a novel tumor-associated neutrophil marker for pancreatic ductal adenocarcinoma.

Zeng Y, Yu J, Chen Y, Zhuang J, Liang X, Li Y, Chen S, Pang W, Zeng L
NPJ precision oncology 2025 Aug 20;9(1):293

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Macrophage migration inhibitory factor in the mouse hippocampus promotes neuroinflammation and cognitive dysfunction following anesthesia and surgery.

Zeng L, Hu PC, Zhang Y, Han TT, Wang X, Zhao YL, Li SY, Luo AL
International immunopharmacology 2025 Apr 4;151:114351

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Tumoral EIF4EBP1 regulates the crosstalk between tumor-associated macrophages and tumor cells in MRTK.

Wu X, Mi T, Jin L, Ren C, Wang J, Zhang Z, Liu J, Wang Z, Guo P, He D
European journal of pharmacology 2024 Sep 5;978:176787

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BV2 Microglial Cell Activation/Polarization Is Influenced by Extracellular Vesicles Released from Mutated SOD1 NSC-34 Motoneuron-like Cells.

Carata E, Muci M, Mariano S, Panzarini E
Biomedicines 2024 Sep 11;12(9)

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Single-cell RNA sequencing reveals the MIF/ACKR3 receptor-ligand interaction between neutrophils and nucleus pulposus cells in intervertebral disc degeneration.

Zhang TL, Chen WK, Huang XP, Zheng BW, Wu PF, Zheng BY, Jiang LX, Escobar D, Li J, Lv GH, Huang W, Zhou H, Xu Z, Zou MX
Translational research : the journal of laboratory and clinical medicine 2024 Oct;272:1-18

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Strategies for arterial graft optimization at the single-cell level.

Hu Z, Dai M, Chang Y, Hua X, Zhang N, Chen X, Sheng Y, Xu Z, Zhang H, Zhang Y, Cui H, Jia H, Wang XJ, Song J
Nature cardiovascular research 2024 May;3(5):541-557

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Extracellular Vesicles from NSC-34 MN-like Cells Transfected with Mutant SOD1 Modulate Inflammatory Status of Raw 264.7 Macrophages.

Carata E, Muci M, Mariano S, Di Giulio S, Nigro A, Romano A, Panzarini E
Genes 2024 Jun 3;15(6)

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EAF2 Downregulation Recruits Tumor-associated Macrophages in Prostate Cancer through Upregulation of MIF.

Cao T, Sun Q, Shi X, Lin X, Lin Q, Zhu J, Xu J, Cui D, Shi Y, Jing Y, Guo W
Biological procedures online 2024 Jul 5;26(1):21

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Multiscale photocatalytic proximity labeling reveals cell surface neighbors on and between cells.

Lin Z, Schaefer K, Lui I, Yao Z, Fossati A, Swaney DL, Palar A, Sali A, Wells JA
Science (New York, N.Y.) 2024 Jul 19;385(6706):eadl5763

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DPSCs regulate epithelial-T cell interactions in oral submucous fibrosis.

Wang SY, Zhang SJ, Meng HF, Xu HQ, Guo ZX, Yan JF, Gao JL, Niu LN, Wang SL, Jiao K
Stem cell research & therapy 2024 Apr 23;15(1):113

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Inhibition of IGF2BP1 attenuates renal injury and inflammation by alleviating m6A modifications and E2F1/MIF pathway.

Mao Y, Jiang F, Xu XJ, Zhou LB, Jin R, Zhuang LL, Juan CX, Zhou GP
International journal of biological sciences 2023;19(2):593-609

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Exercise prevents fatal stress-induced myocardial injury in obese mice.

Dun Y, Hu Z, You B, Du Y, Zeng L, Zhao Y, Liu Y, Wu S, Cui N, Yang F, Liu S
Frontiers in endocrinology 2023;14:1223423

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The crosstalk between M1 macrophage polarization and energy metabolism disorder contributes to polystyrene nanoplastics-triggered testicular inflammation.

Li S, Liu L, Luo G, Yuan Y, Hu D, Xiao F
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2023 Oct;180:114002

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Targeted Knockdown of Macrophage Migration Inhibitory Factor Enhances UVB Irradiation-Induced Apoptosis Via Increasing ROS Generation in Oral Squamous Cell Carcinoma.

Chen T, Chen Q, Li F, Zeng M, Wang B, Huang S, Chen S, Tao Z
Technology in cancer research & treatment 2023 Jan-Dec;22:15330338231163436

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Macrophage migration inhibitory factor (MIF) inhibitor iSO-1 promotes staphylococcal protein A-induced osteogenic differentiation by inhibiting NF-κB signaling pathway.

Shi X, Wu Y, Ni H, Li M, Qi B, Xu Y
International immunopharmacology 2023 Feb;115:109600

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Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment.

Lu X, Luo Y, Nie X, Zhang B, Wang X, Li R, Liu G, Zhou Q, Liu Z, Fan L, Hotaling JM, Zhang Z, Bo H, Guo J
Nature communications 2023 Dec 20;14(1):8462

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Downregulation of macrophage migration inhibitory factor attenuates NLRP3 inflammasome mediated pyroptosis in sepsis-induced AKI.

Li T, Sun H, Li Y, Su L, Jiang J, Liu Y, Jiang N, Huang R, Zhang J, Peng Z
Cell death discovery 2022 Feb 14;8(1):61

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TSP50 promotes hepatocyte proliferation and tumour formation by activating glucose-6-phosphate dehydrogenase (G6PD).

Zhang X, Gao F, Ai H, Wang S, Song Z, Zheng L, Wang G, Sun Y, Bao Y
Cell proliferation 2021 Apr;54(4):e13015

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Proteomic analysis of human periodontal ligament cells under hypoxia.

Li Q, Luo T, Lu W, Yi X, Zhao Z, Liu J
Proteome science 2019;17:3

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Macrophage migration inhibitory factor promotes the invasion and metastasis of oral squamous cell carcinoma through matrix metalloprotein-2/9.

Wang SS, Zheng M, Pang X, Zhang M, Yu XH, Wu JB, Gao XL, Wu JS, Yang X, Tang YJ, Tang YL, Liang XH
Molecular carcinogenesis 2019 Oct;58(10):1809-1821

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Macrophage migration inhibitory factor promotes tumor aggressiveness of esophageal squamous cell carcinoma via activation of Akt and inactivation of GSK3β.

Liu RM, Sun DN, Jiao YL, Wang P, Zhang J, Wang M, Ma J, Sun M, Gu BL, Chen P, Liu K, Ma H, Gao SG, Ma YF, Qi YJ
Cancer letters 2018 Jan 1;412:289-296

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Macrophage migration inhibitory factor promotes vasculogenic mimicry formation induced by hypoxia via CXCR4/AKT/EMT pathway in human glioblastoma cells.

Guo X, Xu S, Gao X, Wang J, Xue H, Chen Z, Zhang J, Guo X, Qian M, Qiu W, Li G
Oncotarget 2017 Oct 6;8(46):80358-80372

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A novel strategy of profiling the mechanism of herbal medicines by combining network pharmacology with plasma concentration determination and affinity constant measurement.

Chen L, Lv D, Wang D, Chen X, Zhu Z, Cao Y, Chai Y
Molecular bioSystems 2016 Oct 18;12(11):3347-3356

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Global secretome characterization of A549 human alveolar epithelial carcinoma cells during Mycoplasma pneumoniae infection.

Li S, Li X, Wang Y, Yang J, Chen Z, Shan S
BMC microbiology 2014 Feb 7;14:27

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 20415-1-AP(MIF antibody) at dilution of 1:500

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human lung using 20415-1-AP(MIF antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The MIF antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human MIF. This antibody recognizes human antigen. The MIF antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

20415-1-AP.pdf

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