55133-1-AP

antibody from Proteintech Group

Targeting: S1PR1 CD363, D1S3362, edg-1, EDG1

Provider product page for 55133-1-AP

Western blot

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Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [67]
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Validations
Western blot [1]

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Product number: 55133-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#55133-1-AP, RRID:AB_10793721
Product name: S1PR1/EDG1 antibody
Antibody type: Polyclonal
Description: KD/KO validated S1PR1/EDG1 antibody (Cat. #55133-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IP, WB, ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

S1PR1 protein structure - 55133-1-AP shown in red.

Submitted references Sphingosine‑1‑phosphate receptor 1 enhances olfactory receptor 51E1‑mediated inhibition of proliferation via Src/JNK signaling in prostate cancer cells.

Kim K, Lee J, Choi C, Bae J, Choi HJ, Huh WK
Oncology reports 2026 May;55(5)

Sphingosine kinase 1 is integral for elastin deficiency-induced arterial hypermuscularization.

Saito J, Dave JM, Gallardo-Vara E, Sadagopan N, Kabir I, Tellides G, Riemer RK, Urban Z, Spiegel S, Hla T, Greif DM
Nature cardiovascular research 2026 Jan;5(1):34-50

Plantaginis Semen, a Novel Natural S1PR1 Agonist, Inhibits Oxidative Stress and Inflammation to Treat Lipopolysaccharide-Induced Acute Lung Injury.

Xu W, Wu J, Lu M, Yu H, Li C
Phytotherapy research : PTR 2026 Jan;40(1):351-369

Cry2 deficiency leads to cognitive impairment through the microbiota-gut-brain axis mediated S1P/NLRP3/IL-1β pathway in mice.

Geng F, Zhao N, Zhou L, Liu XT, Chen X, Wang ZT, Zhang ZJ, Ren QG
Journal of neuroinflammation 2026 Jan 26;23(1):71

Fractional carbon dioxide laser-induced photothermal activation of mesenchymal stem cell-derived exosomes accelerates diabetic wound healing by enhancing angiogenesis.

Chen JY, Ji Z, Guo K, Wang HN, Zhu CC, Li T, Zhao XB, Wang YT, Li Q, Jin PS, Li XY
World journal of diabetes 2026 Jan 15;17(1):112942

Gut microbial metabolite, sphingosine-1-phosphate (S1P), drives mesangial cell phenotypic transformation and accelerates progression of IgA nephropathy via CCL2-MET-FAK pathway.

Tang YY, Zhang DL, Huang LS, Hu P, Liu P, Wu JJ, Xie T, Sun WQ, Xu XD, Jin MP, He HD
Current research in microbial sciences 2025;9:100494

Tumor cell-derived hyaluronan fragments induce endocytosis of S1PR1 to promote lymphangiogenesis through LYVE-1-Src pathway.

Jiang M, Chen D, Xu Z, Liu Y, Yang C, Zhang G, Guo Q, Gao F, He Y, Du Y
Journal of Cancer 2025;16(5):1466-1478

The Combination of PF-543 and TRAIL Effectively Induces Apoptotic Cell Death and Inhibits Stem Cell-Like Properties Through the SPHK1/S1PR1/STAT3 Pathway in TRAIL-Resistant Colorectal Cancer Cells.

Kim SL, Shin MW, Jin BC, Seo SY, Kim SW
Digestive diseases and sciences 2025 Sep;70(9):3041-3055

Imaging and targeting S1PR1 in HER2+ tumors.

Shmuel S, Qiu L, Vanover A, Chen H, Jiang H, Tu Z, Pereira PMR
Nuclear medicine and biology 2025 Sep-Oct;148-149:109057

Ginsenoside F4 inhibits colorectal cancer progression by boosting dendritic cell maturation and remodeling the tumor microenvironment.

Xie W, Li XJ, Zhong YS, Fang J, Qi H, Yang M, Ying HZ, Yu CH
World journal of gastrointestinal oncology 2025 Sep 15;17(9):108892

PET Study of S1PR1 Expression in Rodent Model of Myocardial Infarction.

Chen H, Qiu L, Jiang H, Zhou W, Soda AK, Kovacs A, Weinheimer CJ, Gropler RJ, Tu Z
Molecular imaging and biology 2025 Oct;27(5):810-818

Mitophagy-mediated S1P facilitates muscle adaptive responses to endurance exercise through SPHK1-S1PR1/S1PR2 in slow-twitch myofibers.

Leng M, Yang F, Zhao J, Xiong Y, Zhou Y, Zhao M, Jia S, Liu L, Zheng Q, Gan L, Ye J, Zheng M
Autophagy 2025 Oct;21(10):2111-2129

The S1PR1-CCN1 axis drives endothelial-to-mesenchymal transition and vascular instability in brain arteriovenous malformations.

Li G, Zhang H, Liu M, Liu Y, Shen S, Li S, Zhu W
European journal of medical research 2025 Nov 25;30(1):1175

Dual Mechanisms of Cognitive Function and Pathological Improvements by the Selective S1PR1/5 Modulator Siponimod in 3xTg-AD Mice.

Tang C, Ding Y, Yang S, Lei X, Zhang M, Xu B, He D
Molecular neurobiology 2025 Nov 22;63(1):133

S1PR3 inhibition impairs cell cycle checkpoint via the AKT/WEE1 pathway in oral squamous cell carcinoma.

Zhou X, Liu J, Chen X, Zhou X, Xu B, Gan G, Chen F
Journal of translational medicine 2025 May 23;23(1):573

FTY720 alleviates D-GalN/LPS-induced acute liver failure by regulating the JNK/MAPK pathway.

Guan J, Wu F, Wu S, Ren Y, Wang J, Zhu H
International immunopharmacology 2025 Jun 5;157:114726

Unveiling XinJia-LuHuang Granules' protective mechanism against atherosclerosis: Integrating network pharmacology, metabolomics, and experimental validation.

Liu X, Liu L, Ren X, Chen Y, Yin M, Xie H, Sun T, Tan Y, Wang S, Xu D, Tang S
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Jul 25;143:156919

Dentin tubules as a long-term sustained release carrier to accelerate bone repair by loading FTY720.

Xie J, Huang H, Xu S, Zhou K, Chen X, Fang J, Zhao F
Journal of controlled release : official journal of the Controlled Release Society 2025 Jan 10;377:446-457

The new anti-angiogenesis perspective of rheumatoid arthritis with geniposide: Reducing the extracellular release of HSP70 in HUVECs.

Wei Y, Li Y, Shu Y, Gan PR, Zhu YL, Xu J, Jiang XM, Xia SL, Wang Y, Wu H
International immunopharmacology 2025 Jan 10;144:113645

SPHK1/S1PR1/PPAR-α axis restores TJs between uroepithelium providing new ideas for IC/BPS treatment.

Zhang J, Ge Q, Du T, Kuang Y, Fan Z, Jia X, Gu W, Chen Z, Wei Z, Shen B
Life science alliance 2025 Feb;8(2)

CEBPD-mediated SGPP2 upregulation via PERK/ER stress in endothelial cells disrupts S1P homeostasis and impairs angiogenesis in chronic endometritis.

Wang Y, Chen X, You G, Yu S, Chen C, Lian R, Diao L, Li Y, Yin T
Journal of translational medicine 2025 Dec 17;24(1):98

In situ-formed immunotherapeutic hydrogel containing sphingosine-1-phosphate for enhanced lung cancer immunotherapy.

Shen H, Deng Q, Chen Z, Zhang Q, Zhou X, Chen Q, Fan J
Science advances 2025 Aug 8;11(32):eadw5001

Sanleng Wan inhibits endometriosis progression by regulating sphingolipid metabolism via the S1P/S1PR1-Akt axis based on serum metabolomics and network pharmacology.

Fu Z, Zhou H, Liu H, Li X, Cao L, Zheng W
Journal of ethnopharmacology 2025 Aug 29;352:120153

Hydroxyl safflower yellow A regulates bone-fat balance in osteoporosis by SphK1/S1P/S1PR signaling pathway.

Xu X, Hu X, Zhou S, Liu J, Su Z, Zheng Z, Luo D
Biochemical pharmacology 2025 Aug 20;242(Pt 1):117258

Single-nucleus RNA sequencing and spatial transcriptomics reveal the mechanism by which Xiaozhiling injection treats internal hemorrhoids.

Ke MH, Huang SY, Lin WG, Xu ZG, Zheng XX, Liu XB, Cheng YM, Li ZF
World journal of gastrointestinal surgery 2025 Apr 27;17(4):103494

Assessment of the Effects of Sphingosine Kinase 1/Sphingosine-1-Phosphate on Microangiogenesis at Rat Myofascial Trigger Points Using Contrast-Enhanced Ultrasonography.

Fang X, Yin Y, Lun H, Liu Y, Zhu S
Journal of pain research 2024;17:609-621

S1P/S1PR1 signaling is involved in the development of nociceptive pain.

Dong D, Yu X, Tao X, Wang Q, Zhao L
Frontiers in pharmacology 2024;15:1407347

A guide to selecting high-performing antibodies for S1PR1 (UniProt ID: P21453) for use in western blot, immunoprecipitation, and immunofluorescence.

Ayoubi R, Fotouhi M, Alende C, González Bolívar S, Southern K, Laflamme C, NeuroSGC/YCharOS/EDDU collaborative group, ABIF consortium
F1000Research 2024;13:792

Vitexin attenuates neuropathic pain by regulating astrocyte autophagy flux and polarization via the S1P/ S1PR1-PI3K/ Akt axis.

Huang K, Ding R, Lai C, Wang H, Fan X, Chu Y, Fang Y, Hua T, Yuan H
European journal of pharmacology 2024 Oct 15;981:176848

Involvement of sphingosine-1-phosphate receptor 1 in pain insensitivity in a BTBR mouse model of autism spectrum disorder.

Fan L, Li Q, Shi Y, Li X, Liu Y, Chen J, Sun Y, Chen A, Yang Y, Zhang X, Wang J, Wu L
BMC medicine 2024 Nov 4;22(1):504

CD8(+) tissue-resident memory T cells are essential in bleomycin-induced pulmonary fibrosis.

Feng X, Yu F, He XL, Cheng PP, Niu Q, Zhao LQ, Li Q, Cui XL, Jia ZH, Ye SY, Liang LM, Song LJ, Xiong L, Xiang F, Wang X, Ma WL, Ye H
American journal of physiology. Cell physiology 2024 Nov 1;327(5):C1178-C1191

Pharmacodynamics and mechanism of Erigeron breviscapus granules in the treatment of ischemic stroke in mice by regulating sphingolipid metabolism based on metabolomics.

Liu C, Liu K, Tao X, Li W, Zhao S, Dai Q, Yao H, Mu F, Chen S, Li J, Dong T, Duan J, Wei P, Gao F, Xi M
Journal of pharmaceutical and biomedical analysis 2024 May 15;242:116058

A new look at angiogenesis inhibition of geniposide in experimental arthritis by blocking angiopoietin-2 exocytosis.

Gan P, Wu H, Zhu Y, Shu Y, Wei Y
Phytotherapy research : PTR 2024 Mar;38(3):1245-1261

SPHK1/S1P/S1PR pathway promotes the progression of peritoneal fibrosis by mesothelial-mesenchymal transition.

Zhao T, Ding T, Sun Z, Shao X, Li S, Lu H, Yuan JH, Guo Z
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Jan 31;38(2):e23417

Calorie restriction mimetic, resveratrol, attenuates hepatic ischemia and reperfusion injury through enhancing efferocytosis of macrophages via AMPK/STAT3/S1PR1 pathway.

Yao X, Liu Y, Mao M, Yang L, Zhan Q, Xiao J
The Journal of nutritional biochemistry 2024 Apr;126:109587

The coumarin component isofraxidin targets the G-protein-coupled receptor S1PR1 to modulate IL-17 signaling and alleviate ulcerative colitis.

Huang Y, Chen X, Liu X, Lin C, Wang Y
International immunopharmacology 2024 Apr 20;131:111814

Proximal tubular MBD2 promotes autophagy to drive the progression of AKI caused by vancomycin via regulation of miR-597-5p/S1PR1 axis.

Guo Y, Wu D, Li X, Wang J, Li H, Li Y, Luo D, Yi F, Zhang D
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Apr 15;38(7):e23562

Emilin2 fosters vascular stability by promoting pericyte recruitment.

Fejza A, Camicia L, Carobolante G, Poletto E, Paulitti A, Schinello G, Di Siena E, Cannizzaro R, Iozzo RV, Baldassarre G, Andreuzzi E, Spessotto P, Mongiat M
Matrix biology : journal of the International Society for Matrix Biology 2023 Sep;122:18-32

Cancer/testis antigen CAGE mediates osimertinib resistance in non-small cell lung cancer cells and predicts poor prognosis in patients with pulmonary adenocarcinoma.

Yeon M, Lee H, Yeo J, Jeong MS, Jung HS, Lee H, Shim K, Jo H, Jeon D, Koh J, Jeoung D
Scientific reports 2023 Sep 21;13(1):15748

Estrogen increases the expression of BKCa and impairs the contraction of colon smooth muscle via upregulation of sphingosine kinase 1.

Wang Y, Jiang Y, Jiang L, Xiong W, Wang Y, Gao X, Chen Q, Lin L, Yu T, Tang Y
Journal of cellular physiology 2023 Oct;238(10):2390-2406

TiO(2) nanoparticles promote tumor metastasis by eliciting pro-metastatic extracellular vesicles.

Mu X, Hu K, Wei A, Bai J, Feng L, Jiang J
Journal of nanobiotechnology 2023 Oct 27;21(1):392

S1PR1 serves as a viable drug target against pulmonary fibrosis by increasing the integrity of the endothelial barrier of the lung.

Hao M, Fu R, Tai J, Tian Z, Yuan X, Chen Y, Wang M, Jiang H, Ji M, Lai F, Xue N, Bai L, Zhu Y, Lv X, Chen X, Jin J
Acta pharmaceutica Sinica. B 2023 Mar;13(3):1110-1127

A novel co-target of ACY1 governing plasma membrane translocation of SphK1 contributes to inflammatory and neuropathic pain.

Liu B, Wu W, Cui L, Zheng X, Li N, Zhang X, Duan G
iScience 2023 Jun 16;26(6):106989

Isorhamnetin attenuates the proliferation, invasion, migration and fibrosis of keloid fibroblasts by targeting S1PR1.

Pu X, Cao X, Liu H, Huang W, Zhang L, Jiang T
Experimental and therapeutic medicine 2023 Jul;26(1):310

Gastrodin synergistically increases migration of interleukin-13 receptor α2 chimeric antigen receptor T cell to the brain against glioblastoma multiforme: A preclinical study.

Huang S, Bai Y, An Z, Xu C, Zhang C, Wang F, Zhong C, Zhong X
Phytotherapy research : PTR 2023 Dec;37(12):5947-5957

A comprehensive multiomics approach reveals that high levels of sphingolipids in cardiac cachexia adipose tissue are associated with inflammatory and fibrotic changes.

Qu Y, Wang Y, Wu T, Liu X, Wang H, Ma D
Lipids in health and disease 2023 Dec 1;22(1):211

Sphingosine 1-phosphate receptor 1 regulates blood-brain barrier permeability in epileptic mice.

Yang LX, Yao YY, Yang JR, Cheng HL, Zhu XJ, Zhang ZJ
Neural regeneration research 2023 Aug;18(8):1763-1769

Fingolimod protects against neurovascular unit injury in a rat model of focal cerebral ischemia/reperfusion injury.

Zhu XY, Ma TT, Li Y, Zhang MQ, Zhao L, Liang J, Min LQ
Neural regeneration research 2023 Apr;18(4):869-874

Dexmedetomidine alleviates oxidative stress and mitochondrial dysfunction in diabetic peripheral neuropathy via the microRNA-34a/SIRT2/S1PR1 axis.

Lin Y, Wei Y, Wei Y, Yu H, Zhang W, Li C, He Y, Yao G, Zhang Y
International immunopharmacology 2023 Apr;117:109910

Global sphingosine-1-phosphate receptor 2 deficiency attenuates neuroinflammation and ischemic-reperfusion injury after neonatal stroke.

Fukuzaki Y, Faustino J, Lecuyer M, Rayasam A, Vexler ZS
iScience 2023 Apr 21;26(4):106340

Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation.

Wang Y, Wu H, Gui BJ, Liu J, Rong GX, Deng R, Bu YH, Zhang H
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Jun;100:154068

Crosstalk between pro-survival sphingolipid metabolism and complement signaling induces inflammasome-mediated tumor metastasis.

Janneh AH, Kassir MF, Atilgan FC, Lee HG, Sheridan M, Oleinik N, Szulc Z, Voelkel-Johnson C, Nguyen H, Li H, Peterson YK, Marangoni E, Saatci O, Sahin O, Lilly M, Atkinson C, Tomlinson S, Mehrotra S, Ogretmen B
Cell reports 2022 Dec 6;41(10):111742

Potential of a sphingosine 1-phosphate receptor antagonist and sphingosine kinase inhibitors as targets for multiple myeloma treatment.

Tanaka Y, Okabe S, Ohyashiki K, Gotoh A
Oncology letters 2022 Apr;23(4):111

The CAGE-MiR-181b-5p-S1PR1 Axis Regulates Anticancer Drug Resistance and Autophagy in Gastric Cancer Cells.

Yeon M, Kim Y, Pathak D, Kwon E, Kim DY, Jeong MS, Jung HS, Jeoung D
Frontiers in cell and developmental biology 2021;9:666387

Inhibition of Sphingosine-1-Phosphate Receptor 2 Prevents Thoracic Aortic Dissection and Rupture.

Pan G, Liao M, Dai Y, Li Y, Yan X, Mai W, Liu J, Liao Y, Qiu Z, Zhou Z
Frontiers in cardiovascular medicine 2021;8:748486

Interference with the expression of S1PR1 or STAT3 attenuates valvular damage due to rheumatic heart disease.

Xian S, Chen A, Wu Y, Wen H, Lu C, Huang F, Zeng Z
International journal of molecular medicine 2021 Sep;48(3)

Inhibition of sphingosine 1-phosphate (S1P) receptor 1/2/3 ameliorates biological dysfunction in rheumatoid arthritis fibroblast-like synoviocyte MH7A cells through Gαi/Gαs rebalancing.

Wang M, Wu H, Wang R, Dai X, Deng R, Wang Y, Bu Y, Sun M, Zhang H
Clinical and experimental pharmacology & physiology 2021 Aug;48(8):1080-1089

Geniposide downregulates the VEGF/SphK1/S1P pathway and alleviates angiogenesis in rheumatoid arthritis in vivo and in vitro.

Wang Y, Wu H, Deng R, Dai XJ, Bu YH, Sun MH, Zhang H, Wang MD, Wang RH
Phytotherapy research : PTR 2021 Aug;35(8):4347-4362

Bone marrow mesenchymal stem cells-derived exosomes reduce Aβ deposition and improve cognitive function recovery in mice with Alzheimer's disease by activating sphingosine kinase/sphingosine-1-phosphate signaling pathway.

Wang X, Yang G
Cell biology international 2021 Apr;45(4):775-784

Coix Seed Oil Exerts an Anti-Triple-Negative Breast Cancer Effect by Disrupting miR-205/S1PR1 Axis.

Fang T, Jiang YX, Chen L, Huang L, Tian XH, Zhou YD, Nagle DG, Zhang DD
Frontiers in pharmacology 2020;11:529962

Combination of sphingosine-1-phosphate receptor 1 (S1PR1) agonist and antiviral drug: a potential therapy against pathogenic influenza virus.

Zhao J, Zhu M, Jiang H, Shen S, Su X, Shi Y
Scientific reports 2019 Mar 27;9(1):5272

S1P promotes inflammation-induced tube formation by HLECs via the S1PR1/NF-κB pathway.

Zheng Z, Zeng YZ, Ren K, Zhu X, Tan Y, Li Y, Li Q, Yi GH
International immunopharmacology 2019 Jan;66:224-235

Hepatocyte-Derived Lipotoxic Extracellular Vesicle Sphingosine 1-Phosphate Induces Macrophage Chemotaxis.

Liao CY, Song MJ, Gao Y, Mauer AS, Revzin A, Malhi H
Frontiers in immunology 2018;9:2980

Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.

Pang HQ, Yue SJ, Tang YP, Chen YY, Tan YJ, Cao YJ, Shi XQ, Zhou GS, Kang A, Huang SL, Shi YJ, Sun J, Tang ZS, Duan JA
Frontiers in pharmacology 2018;9:165

Sphingosine 1-phosphate (S1P) reduces hepatocyte growth factor-induced migration of hepatocellular carcinoma cells via S1P receptor 2.

Matsushima-Nishiwaki R, Yamada N, Fukuchi K, Kozawa O
PloS one 2018;13(12):e0209050

Sphingosine-1-phosphate mediates AKT/ERK maintenance of dental pulp homoeostasis.

Pan HY, Yang H, Shao MY, Xu J, Zhang P, Cheng R, Hu T
International endodontic journal 2015 May;48(5):460-8

H9N2 avian influenza infection altered expression pattern of sphiogosine-1-phosphate receptor 1 in BALB/c mice.

Tong S, Tian J, Wang H, Huang Z, Yu M, Sun L, Liu R, Liao M, Ning Z
Virology journal 2013 Sep 30;10:296

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The S1PR1 /EDG1 antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human S1PR1 /EDG1. This antibody recognizes human, mouse antigen. The S1PR1 /EDG1 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 55133-1-AP.pdf

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