AM08433PU-S

antibody from Acris Antibodies GmbH

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM08433PU-S

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM08433PU-S, RRID:AB_2035100

Product name

anti AKT1 / PKB pThr308

Antibody type

Monoclonal

Antigen

Synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

18F3.H11

Vial size

25 µl

Concentration

1,48 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Anti-AKT pT308 monoclonal antibody shows detection of phosphorylated AKT (indicated by arrowhead at ~56 kDa) on PDGF stimulated NIH/3T3 cell lysates (lane 2). No reactivity is seen for non-phosphorylated AKT in untreated cells (lane 1). Each lane contained approximately 15 µg of lysate. All samples were loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 3% BSA in TBS for 30 min at RT. The membrane was probed with the primary antibody at a 1:4,000 dilution in TBS with 3% BSA, for 3 h at 4° C. For detection peroxidase conjugated Gt-a-Mouse IgG (Fc) was used at a 1:40,000 dilution for 1 h at 4° C. Data was captured using a Versadoc 4000 MP (Bio-Rad).

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry using Anti-AKT pT308 monoclonal antibody shows detection of phosphorylated AKT pT308 in human brain cerebellum tissue (40X). The antibody was used a dilution to 20 µg/mL. The image shows strong staining of Purkinje neurons and cell processes in the cerebellum. Staining was both cytosolic as well as occasionally nuclear, and the antibody showed minimal background staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.