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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- AP22624SU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP22624SU-N, RRID:AB_10850419
- Product name
- anti AKT1/AKT2/AKT3 (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide -KLH conjugated corresponding to the C-terminus (460-480) of Human, Rat, Mouse and Chicken AKT proteins conjugated to KLH using maleimide.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Rabbit
- Vial size
- 50 µl
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP22624PU-N AKT antibody staining at 1/500 dilution by Immunoblotting.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence Microscopy: Rabbit anti-AKT antibody was used at a 1/80 dilution to stain cultured neonatal Rat cardiomyocytes that express a nuclear-targeted AKT construct. Anti-AKT staining appears green. Actin filaments are labeled red using a Texas-red⢠conjugated phalloidin.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP22624PU-N AKT antibody staining of Formalin-Fixed Paraffin-Embedded Human Liver at 1/100 followed by Biotin conjugated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP22624PU-N AKT antibody staining of Formalin-Fixed Paraffin-Embedded Sections at 1/1,000 dilution: Panel A: Normal colon tissue Panel B: Tumor tissue.
