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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- R1464 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1464, RRID:AB_972612
- Product name
- anti AKT1/AKT2/AKT3 (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to the C-terminus (460-480) of Human, Rat and Mouse and chicken AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Rabbit
- Vial size
- 0.2 ml
- Concentration
- 75 mg/ml (by refractometry)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoblotting. R1464 AKT antibody was used at 1/500 dilution to detect AKT by Western blot. Approximately 20 µg of an NIH/3T3 whole cell lysate was loaded on a 10% NuPage SDS-PAGE gel using MOPS buffer. After washing, a 1/10,000 dilution of HRP conjugated Goat anti-Rabbit IgG (R1454HRP) preceded color development using Pierce Chemical's SuperSignal substrate.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence Microscopy: R1464 AKT antibody was used at 1/80 dilution to stain cultured neonatal Rat cardiomyocytes that express a nuclear-targeted AKT construct. Anti-AKT staining appears green. Actin filaments are labeled red using a Texas-red conjugated phalloidin.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry: R1464 AKT antibody was used at 1/1,000 dilution to detect AKT by Immunohistochemistry. In panel (A) normal colon tissue is stained. Panel (B) shows tumor tissue. Tissue was Formalin-Fixed and Paraffin Embedded.
