AP50137PU-N

antibody from Acris Antibodies GmbH

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

AP50137PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP50137PU-N, RRID:AB_11147190

Product name

anti AKT1 / PKB (C-term)/(C-term T450)

Antibody type

Polyclonal

Antigen

KLH conjugated synthetic peptide selected from the C-terminal region of human AKT1

Reactivity

Human, Mouse

Host

Rabbit

Vial size

0.4 ml

Concentration

lot specific

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AKT1 Antibody (pT450) (Cat. #AP50137PU-N) western blot analysis in MCF-7 cell line lysates (35 µg/lane).This demonstrates the AKT1 antibody detected the AKT1 protein (arrow).

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of AKT1 Antibody (C-term T450)(Cat#AP50137PU-N) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AKT1 Antibody (C-term T450) (Cat. #AP50137PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AKT1 Antibody (C-term T450) for immunohistochemistry. Clinical relevance has not been evaluated.