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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AM00090PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM00090PU-N, RRID:AB_1004974
- Product name
- anti MAP2K1 (incl. pos. control)
- Antibody type
- Monoclonal
- Antigen
- Phosphopeptide conjugated to KLH.Epitope: pSer 218/222 in MEK1 pSer 222/226 in MEK2
- Reactivity
- Human, Mouse, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7E10
- Vial size
- 0.1 mg
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Phosphospecificity: Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab MEK1/2-7E10 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
