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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- SP7116P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SP7116P, RRID:AB_1004975
- Product name
- anti MAP2K1 / MAP2K2 pSer218/222
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Ser218/222 of human MEK 1/2
- Reactivity
- Human, Mouse, Rat, Xenopus
- Host
- Rabbit
- Vial size
- 0.1 ml
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western Blot of NIH 3T3 cell lysates. The cells were either serum starved (Control) or incubated in the presence of serum for 5 minutes. The Western blot shows the immunolabeling of the ~45 kDa MEK 1/2 was absent in controls (Lane A) and stimulated by serum (Lane B). Immunolabeling of an additional band at ~100 kDa was also seen.
