AM00095PU-N

antibody from Acris Antibodies GmbH

Targeting: MAP2K5 HsT17454, MAPKK5, MEK5, PRKMK5

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AM00095PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00095PU-N, RRID:AB_1004990

Product name

anti MAP2K5 (incl. pos. control)

Antibody type

Monoclonal

Antigen

Synthetic peptide conjugated to hemocyanin

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

14B5

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Detection of endogenous MKK5 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab MKK5-14B5 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2