Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP31816PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP31816PU-N, RRID:AB_11148517
- Product name
- anti Neuron specific enolase
- Antibody type
- Polyclonal
- Antigen
- Chickens were immunized with two synthetic peptide KLH conjugates corresponding to different regions of the NSE-2 gene product, but are shared between the Human (NP_001966, NCBI) and Rat (AAA41119) sequences.Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of each of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
- Reactivity
- Human, Mouse, Rat
- Host
- Chicken/Avian
- Vial size
- 0.2 mg
- Concentration
- The concentrations of each of the two antibodies was 0.1 mg/ml (based on Bradford assay readings using BSA as a standard), making the total antibody concentration 0.2 mg/ml.
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Dissociated cell cultures of neonatal Mouse brains, showing NSE-2 (green staining) in neurons. These cultures were counter-stained with a Rabbit anti-GFAP antibody to localize astrocytes, as well as with DAPI (blue staining) to localize nuclei.
