SM5081

antibody from Acris Antibodies GmbH

Targeting: NFATC1 NF-ATC, NFAT2, NFATc

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunoelectron microscopy (Recommended by provider)

Gel shift (Recommended by provider)

Antibody Data

Product number

SM5081

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#SM5081, RRID:AB_1005665

Product name

anti NFAT2 / NFATC (1-654)

Antibody type

Monoclonal

Antigen

Bacterially expressed glutathione S-transferase (GST) fusion protein containing NF-ATc1 residues 1-654.

Reactivity

Human, Mouse, Rat, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

7A6

Vial size

0.1 ml

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescent analysis of NFATc1 using NFATc1 Monoclonal Antibody (7A6) (Cat.-No SM5081) shows staining in MCF-7 Cells. NFATc1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc1 (Cat.-No SM5081) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescent analysis of NFATc1 using NFATc1 Monoclonal Antibody (7A6) (Cat.-No SM5081) shows staining in Hela Cells. NFATc1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc1 (Cat.-No SM5081) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescent analysis of NFATc1 using NFATc1 Monoclonal Antibody (7A6) (Cat.-No SM5081) shows staining in 293Cells. NFATc1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc1 (Cat.-No SM5081) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.