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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AP21607SU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP21607SU-S, RRID:AB_10757405
- Product name
- anti Bax
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to amino acids 43-61 (PELALDPVPQDASTKKLSE) of human Bax alpha
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 50 µl
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of BAX in colon tumor cell lines using AP21607SU-S. Bax is detected in the SW620 and DLD-1 cell lines. However, Bax was not detected in the cell lines (LoVo, DU145, and LS180) known to have frameshift mutations in Bax (Rampino et al. 1997). An antibody to F1BATPase was used as a protein loading control.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded section of a human colon cancer tissue microarray stained for BAX using AP21607SU-S at 1/2000. Hematoxylin-eosin counterstain.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded sections of human colon cancer fom a tissue microarray stained for BAX using AP21607SU-S at 1/2000. A. High Bax expression. B. Low Bax expression. Hematoxylin-eosin counterstain.
