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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- GTX48650 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48650, RRID:AB_11174995
- Product name
- ATM (phospho Ser1981) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Sheep
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- anti-ATM polyclonal antibody was used in western blot. Lane 2 shows anti-ATM pS1981 polyclonal antibody detects ATM in Hydrogen Peroxide Stimulated Human Derived MCF-7 Whole Cell Lysate
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western Blot of Sheep Anti-ATM pS1981 polyclonal antibody. Lane 1: untreated MCF-7 cell lysate. Lane 2: Hydrogen Peroxide stimulated MCF-7 Whole Cell Lysate. Load: 35 ?g per lane. Primary antibody: ATM pS1981 antibody at 1:1000 for 1 h at room temperature. Secondary antibody: IRDye?800 conjugated Donkey anti-Sheep IgG secondary antibody at 1:5,000 for 1h at room temperature. Block: 5% BLOTTO overnight at 4¢XC. Predicted/Observed size: 370 kDa, ATM (370 kDa) is indicated by an arrow. Other band(s): ATM splice variants and isoforms.
