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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Flow cytometry [3]
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Validation data
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- Product number
- GTX84977 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84977, RRID:AB_10728270
- Product name
- ACAT2 antibody [1C5]
- Antibody type
- Monoclonal
- Reactivity
- Human, Rat, Canine, Simian
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-ACAT2 mouse monoclonal antibody (GTX84977) immunofluorescent staining of COS7 cells transiently transfected with ACAT2
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-ACAT2 mouse monoclonal antibody (GTX84977).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-ACAT2 antibody(GTX84977),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-ACAT2 antibody(GTX84977),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201821 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACAT2 antibody(GTX84977), and then analyzed by flow cytometry.
