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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Proximity ligation assay [1]
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- Product number
- H00000369-M05 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000369-M05, RRID:AB_489875
- Product name
- ARAF monoclonal antibody (M05), clone 3E2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant ARAF.
- Antigen sequence
RQQFYHSVQDLSGGSRQHEAPSNRPLNELLTPQGP
SPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHM
VSTTAPMDSNLIQLTGQSFSTDAAGSRGGS- Isotype
- IgG
- Antibody clone number
- 3E2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ARAF monoclonal antibody (M05), clone 3E2 Western Blot analysis of ARAF expression in HeLa ( Cat # L013V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ARAF expression in transfected 293T cell line by ARAF monoclonal antibody (M05), clone 3E2.Lane 1: ARAF transfected lysate(67.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ARAF is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between PKM2 and ARAF. HeLa cells were stained with anti-PKM2 rabbit purified polyclonal 1:1200 and anti-ARAF mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
