H00000699-M02

antibody from Abnova Corporation

Targeting: BUB1 BUB1A, BUB1L, hBUB1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000699-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000699-M02, RRID:AB_464015

Product name

BUB1 monoclonal antibody (M02), clone 2F9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant BUB1.

Antigen sequence

MDTPENVLQMLEAHMQSYKGNDPLGEWERYIQWVE
ENFPENKEYLITLLEHLMKEFLDKKKYHNDPRFIS
YCLKFAEYNSDLHQFFEFLYNHGIGTLSSPLYIAW
AGHLEAQGELQHASAVLQRGIQNQA


Isotype

IgG

Antibody clone number

2F9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

BUB1 monoclonal antibody (M02), clone 2F9 Western Blot analysis of BUB1 expression in Hela S3 NE ( Cat # L013V3 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of BUB1 expression in transfected 293T cell line by BUB1 monoclonal antibody (M02), clone 2F9.Lane 1: BUB1 transfected lysate(122 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged BUB1 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of BUB1 transfected lysate using anti-BUB1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with BUB1 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol