H00000207-M04

antibody from Abnova Corporation

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000207-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000207-M04, RRID:AB_490106

Product name

AKT1 monoclonal antibody (M04), clone 6D6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant AKT1.

Antigen sequence

MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTF
IGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRP
NTFIIRCLQWTTVIERTLHVETPEEREEWTTAIQT
VADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAK
PKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRY
YAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFL
TALKYSFQTHDRLCFVMEYANGGELFFHLSRERVF
SEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENL
MLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEY
LAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFY
NQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLK
KDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKK
LSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQD
DSMECVDSERRPHFPQFSYSASGTA


Isotype

IgG

Antibody clone number

6D6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

AKT1 monoclonal antibody (M04), clone 6D6 Western Blot analysis of AKT1 expression in HeLa ( Cat # L013V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged AKT1 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to AKT1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol