H00000208-M05

antibody from Abnova Corporation

Targeting: AKT2 PKBβ

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000208-M05

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000208-M05, RRID:AB_893996

Product name

AKT2 monoclonal antibody (M05), clone 1F3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant AKT2.

Antigen sequence

MRAIQMVANSLKQRAPGEDPMDYKCGSPSDSSTTE
EMEVAVSKARAKVTMNDFDYLKLLGKGTFGKVILV
REKATGRYYAMKILRKEVII


Isotype

IgG

Antibody clone number

1F3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

AKT2 monoclonal antibody (M05), clone 1F3 Western Blot analysis of AKT2 expression in Jurkat ( Cat # L017V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

AKT2 monoclonal antibody (M05), clone 1F3. Western Blot analysis of AKT2 expression in Raw 264.7(Cat # L024V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged AKT2 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to AKT2 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol