MAB0065

antibody from Abnova Corporation

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB0065

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB0065, RRID:AB_1019091

Product name

AKT1 (phospho S473) monoclonal antibody, clone 104A282

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against synthetic phosphopeptide of AKT1.

Isotype

IgG

Antibody clone number

104A282

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Deguelin, an Akt inhibitor, suppresses IkappaBalpha kinase activation leading to suppression of NF-kappaB-regulated gene expression, potentiation of apoptosis, and inhibition of cellular invasion.

Nair AS, Shishodia S, Ahn KS, Kunnumakkara AB, Sethi G, Aggarwal BB
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5612-22

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of AKT1. Using AKT1 (phospho-S473) monoclonal antibody, clone 104A282 (Cat # MAB0065) at 2 ug/mL against 10 ug/lane of untreated (lane 1) and PDGF treated (lane 2) NIH/3T3 lysate.