H00003932-M01

antibody from Abnova Corporation

Targeting: LCK

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003932-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003932-M01, RRID:AB_425524

Product name

LCK monoclonal antibody (M01), clone 3F7-F5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant LCK.

Antigen sequence

MGCGCSSHPEDDWMENIDVCENCHYPIVPLDGKGT
LLIRNGSEVRDPLVTYEGSNPPASPLQDNLVIALH
SYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTT
GQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQ
LLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQG
EVVKHYKIRNLDNGGFYISPRITFPGLHELVRHYT
NASDGLCTRLSRPCQTQKPQKPWWEDEWEVPRETL
KLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSM
SPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYII
TEYMENDTLLDSQLEEKGLGASPWGNLGQQLLLLP
TGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFI
EERNYIHRDLRAANILVSDTLSCKIADFGLARLIE
DNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWS
FGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMV
RPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLE
DFFTATEGQYQPQP


Isotype

IgG

Antibody clone number

3F7-F5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

LCK monoclonal antibody (M01), clone 3F7-F5 Western Blot analysis of LCK expression in Jurkat ( Cat # L017V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of LCK expression in transfected 293T cell line by LCK monoclonal antibody (M01), clone 3F7-F5.Lane 1: LCK transfected lysate(59.4 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged LCK is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of LCK transfected lysate using anti-LCK monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with LCK MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol